Abstract: The comparative study on degrading of ramie gelatin with enzymes under supercritical CO2 and normal atmosphere was carried out. The changing rule of main degradation product——sugar was analyzed by using DNS, HPLC and carbazole methods. Apparent examination of the degraded remie bast structure was conducted with SEM. The gelatin removal rate reached 17.2% when the pressure of supercritical CO2 was 10 Mpa with 0.4 % of pectinase, 0.2 % of hemicellulase, and the speed of 250 r/min for treating 3 h. Enzymic catalytic reaction was centralized within 2 h under the two systems and was shorter by 0.5 h under supercritical CO2. The highest value of TRS was 763 mg/20 g and, of glucose, 230 mg/20 g.