Abstract: In order to make full use of tropical agricultural biotic resources and make up raw material shortage for textile industry in China,enzymatic degumming of pineapple leaf fibre was explored in both theoretical and practical aspects. Purified pectase with high enzymatic activity from a strain screened by us was used.It was shown that the optimal result of degumming could be achieved when reaction conditions were pectinase dosage 8%,pH value 7.0,(52 ℃) and treated for 4 hours.A post_treatment after degumming with xylanase for 45 min and following treatment with H_2O_2 for 15 min would yield fiber qualified for textile utilization.