纺织学报 ›› 2010, Vol. 31 ›› Issue (8): 56-60.

• 染整与化学品 • 上一篇    下一篇

苎麻微生物脱胶特异菌株的筛选与鉴定

蓝广芊1,左伟东,许平震2,汪涛3,胡光毅3,邓朝海3,吴大洋3   

  1. 1. 重庆西南大学纺织服装学院2. 西南大学生物技术学院3. 西南大学纺织服装学院
  • 收稿日期:2009-07-23 修回日期:2010-03-23 出版日期:2010-08-15 发布日期:2010-08-15
  • 通讯作者: 蓝广芊

screening and Identification of a Bacterium Strain with specific Ramie degumming

Zuo Weidong Zuo,Xu Pingzhen Xu,Hu Guangyi Hu,Wu Dayang Wu   

  • Received:2009-07-23 Revised:2010-03-23 Online:2010-08-15 Published:2010-08-15

摘要: 本论文通过从野外采集土样,采取多次富集、初筛复筛的手段筛选得到一株对苎麻具有较强脱胶能力的菌株,命名为Dazu5-1。利用该菌株对苎麻进行2d发酵脱胶,平均减重率达到30.2%,与化学脱胶相比,经过Dazu5-1菌株脱胶后的苎麻单纤维强力平均值提高76.93%;最后对Dazu5-1菌株进行了形态学观察,生理生化指标测定、16SrDNA碱基序列测定和同源性分析,鉴定该菌株为志贺菌属。

Abstract: In this paper, a bacterium strain with high-efficient ramie degumming , named Dazu5-1, was isolated from field soil by iterative enrichment,screening and rescreening methods. When the strains are used for 2d fermentation degumming of ramie, the average weight loss rate can reach 30.2%. Compared with the chemical degumming, Dazu5-1 strain degumming can make the average value of single fiber strength of ramie increase by 76.93%. Finally, the Dazu5-1 strain is identified as Shigella by morphological observation, physiological and biochemical indexes determination, 16SrDNA base sequencing and homology analysis.

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