纺织学报 ›› 2011, Vol. 32 ›› Issue (6): 1-6.

• 纤维材料 •    下一篇

丝素蛋白/硅酸钙复合纳米纤维的结构与性能

朱海霖1,冯新星2,陈建勇1   

  1. 1. 浙江理工大学
    2. 浙江理工大学材料与纺织学院
  • 收稿日期:2010-06-03 修回日期:2010-08-09 出版日期:2011-06-15 发布日期:2011-06-15
  • 通讯作者: 陈建勇 E-mail:cjy@zstu.edu.cn
  • 基金资助:

    国家级

The structures and properties of electrospun silk fibroin/CaSiO3 composite nanofibers

  • Received:2010-06-03 Revised:2010-08-09 Online:2011-06-15 Published:2011-06-15

摘要: 采用静电纺丝法制备了丝素蛋白/硅酸钙复合纳米纤维材料。X射线衍射(XRD)和傅立叶变换红外光谱(FTIR)分析表明复合纤维材料中丝素的结构主要以Silk II为主;场发射扫描电镜(FESEM)显示复合纤维的直径为200-400nm,孔隙率约为78%,孔隙分布均匀。采用水接触角法测定了材料的亲水性,相比于纯丝素纤维材料,复合纤维材料的亲水性有所提高。分别应用模拟体液和Tris缓冲液浸泡实验研究了复合纳米纤维材料的体外生物活性和降解性,并用XRD和衰减全反射红外光谱(ATR-IR)对试样表面进行了测定。研究表明,20wt%硅酸钙/丝素蛋白复合纳米纤维材料在模拟体液中浸泡7天后表面覆盖了较厚的类骨羟基磷灰石层,其在Tris缓冲液中浸泡12周后的降解率达42.4%,具有良好的生物活性和降解性。研究结果显示丝素蛋白/硅酸钙复合纳米纤维材料有望作为生物活性骨组织修复材料。

Abstract: The silk fibroin/CaSiO3 composite nanofibers with interconnected pore structures were fabricated via electrospinning. X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy analysis showed that the structure of SF was Silk II. The results of field emission scanning electron microscopy (FESEM) showed that the diameters and porosities of the composite nanofibers were 200-400nm and about 78%, respectively. The surface hydrophilicities of the composite nanofibers were improved compared with that of pure SF nanofibers. The samples were soaked in simulated body fluid (SBF) and Tris-buffer to investigate their bioactivities and degradabilities, respectively. After soaking in SBF for 7 days, the surfaces of the composite nanofibers with 20% wt CaSiO3 were covered by hydrocarbonate apatite layer. The degradability of this sample reached 42.4% after soaking in Tris-buffer for 12 weeks. The addition of CaSiO3 into the silk fibroin nanofibers could improve in vitro bioactivity of the composite nanofibers and provide a promising opportunity to widen potential application of silk fibroin in bone tissue engineering.

中图分类号: 

  • TQ342+.87TQ342+.87
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