Abstract: The 9 factors that influenced laccase production from Polyporus elegans Fr. was studied using Plackett-Burman design. The result showed that the main nutrition factors was C/N ratio, the concentration of Cu2+ and pH. On that basis，response surface analysis were adopted to investigate the optimal levels of the 3 main factors. The result was undertaked the second regression analysis using Design-Expert software and showed that the laccase activity was 446U/mL(178U/mL before optimization) when the C/N ratio, the concentration of Cu2+ and pH was 4:1, 0.1 mmol/L and 5.5 respectively.