Abstract: A method for the determination of nitenpyram, dinotefuran, thiamethoxam, clothiandin, imidacloprid, acetamiprid and thiacloprid in cotton samples has been introducted. The isotope internal standard (imidcacloprid-D4) was added into the sample solutions. The sample was extracted with acetonitrile. The ionisation mode used was positive mode with multiple reaction monitoring. The one precursor/two product ion transitions for each neonicotinoids were used. The results show that the working curves were linear in the range of 0~12.5 ug/L. The recoveries were between 75.9-98.4% and the relative standard deviations were less 15.8% at the spiked levels of 10, 20 and 40 ug/kg. The limits of quantitation were 10 ug/kg. The method is simple, rapid, and accurate, and its performance can meet the requirements of the domestic and international legislation. The method adapts to confirm residues of neonicotinoid pesticides in cotton samples.