Abstract:

The bisphenol A in textiles and clothing has low molecular weight, that is kifficult to be accurately measured. The surface plasmon resonance (SPR) and solution competition method was used to detect it. In order to better bind bisphenol A and bovine serum albumin antigens (BPA-BSA) with sensing chips to generate amino groups, four factors including selection of the sensing chip, the modification of pH for the solutions, the ion concentration and the optimal concentration of BPA-BSA were taken into account. By controlling the variates of the four factors, the best condition for sensor chip to detect BPA was edtermined. The results show that the best amino-coupled effect of BPA-BSA occurs using carboxymethyl dextran assembly (CM5) chip at pH 4.5, the ion concentration of 10 mmol/L and the concentration of BPA-BSA of 50μg / mL. The pretreatment method is simple and efficient, and high specificity BPA an detected without precision purification. This method can be used in textiles for BPA detection.

Key words: textile, bisphenol A , surface plasmon resonance, amino coupling, solution competition method