Abstract: A comparative study on the degumming properties of high-efficient ramie strains Erwinia CXJZU-120 and Bacillus subtilis T66 was reported on the optimal conditions. The results showed that: compared with the strain T66, the strain CXJZU-120 could finish ramie degumming without chemical post-processing; CXJZU-120 for ramie degumming could make the the rate of degumming improve by 6.75%, the pollution reduce by 50%; the residue gum content of refined fiber degummed by CXJZU-120 was less than 1%. The research illustrated that the two strains in the degumming properties were obvious differences, and the ramie degumming method could be achieved from chemical field to biological field.