栗生灰黑孔菌合成漆酶最佳发酵条件的优化

纺织学报 ›› 2011, Vol. 32 ›› Issue (12): 64-70.

栗生灰黑孔菌合成漆酶最佳发酵条件的优化

李振光¹,沈明浩²

1. 吉林农业大学
2.

收稿日期:2010-12-01 修回日期:2011-07-01 出版日期:2011-12-15 发布日期:2011-12-16
通讯作者: 李振光 E-mail:xiaoguang2464@126.com
基金资助:
国家级

Received:2010-12-01 Revised:2011-07-01 Online:2011-12-15 Published:2011-12-16

摘要/Abstract

摘要： 本实验研究了碳源、氮源、诱导剂、金属离子、pH等培养条件对栗生灰黑孔菌Melanoporia castanea (Imazeki)T. Hatt. & Ryvarden产漆酶的影响。并利用Minitab15软件、Plackett-Burman PB 实验设计和响应面分析法对栗生灰黑孔菌Melanoporia castanea (Imazeki)T. Hatt. & Ryvarden产漆酶的发酵条件进行优化。结果表明：1.最佳液态发酵培养基组分为：玉米秆粉9.976g/L，黄豆粉9g/L，ZnSO4•7H2O 0.3µmol /L，对苯二胺0.5mmol/L。2、最佳培养条件：温度28℃，转速180r/min，PH值为5.913，装液量60ml/250ml,接种量10%。在以上条件下培养漆酶酶活可达到431 U/ml。

Abstract: The experimental study the optimal culture conditions for laccase production by Melanoporia castanea in shake-flask cultivation And use Minitab15 software, Plackett-Burman PB experimental design and response surface analysis optimize Melanoporia castanea (Imazeki) T. Hatt. & Ryvarden laccase fermentation conditions.The composition of fermentation medium :Corn stalks 9.976g/L, Soybean Meal 9g / L, ZnSO4•7H2O 0.3μmol / L, p-phenylenediamine 0.5mmol / L. Optimal culture conditions: temperature 28 ℃, speed 180r/min, PH 5.913, Liquid volume 60ml/250ml, inoculum 10%. Under the above cultured conditions, laccase activity can be achieved 431U / ml.

李振光, 沈明浩. 栗生灰黑孔菌合成漆酶最佳发酵条件的优化[J]. 纺织学报, 2011, 32(12): 64-70.

LI Zhen-Guang, SHEN Ming-Hao. [J]. JOURNAL OF TEXTILE RESEARCH, 2011, 32(12): 64-70.

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