纺织学报 ›› 2014, Vol. 35 ›› Issue (12): 6-0.

• 纤维材料 • 上一篇    下一篇

聚乳酸∕聚乙醇酸新型纳米支架载药体系研制

  

  • 收稿日期:2013-12-23 修回日期:2014-07-16 出版日期:2014-12-15 发布日期:2014-12-16
  • 通讯作者: 卫彦 E-mail:kqdxl@bjmu.edu.cn

Research on the system of a novel polyglycolic acid nanofibers scaffold loaded drug

  • Received:2013-12-23 Revised:2014-07-16 Online:2014-12-15 Published:2014-12-16

摘要: 将药物盐酸四环素(Tet)与聚乳酸-聚乙醇酸(PLGA)同时溶解在四氢呋喃和N-N二甲基甲酰胺(体积比为1:1)的有机溶液中,通过静电纺丝技术制备载药PLGA/Tet纳米纤维膜。通过扫描电镜(SEM)和荧光显微镜观察纳米纤维膜的形貌结构;用紫外分光光度计检测药物的吸光度,并计算其释药速率;通过改良的Kirby-Bauer纸片扩散法观察其体外抑菌性能;SEM观察细胞和支架的形态、黏附及生长情况。结果显示不同载药量的纳米纤维膜的平均直径在360–470nm之间,无显著差异;载药量为10%的纳米纤维突释最大,在体外可持续释药27天以上。体外抗菌活性结果表明纳米纤维膜对金黄色葡萄球菌均有瞬时、长效的抑菌活性。SEM观察显示人成骨样细胞MG-63与纳米纤维膜在支架上共培养后生长良好,表明其细胞相容性良好。

Abstract: Abstract: Mixing tetracycline hydrochloride (tet) with poly-(D,L-lactide-co-glycolide) (PLGA) in THF and DMF (1:1 v/v),the PLGA nanofibers membrane with tetracycline hydrochloride were prepared by electrospinning. The morphlogy were observed by means of scan electric microscope(SEM) and fluorescence microscopy. The absorbency of drug was detected by UV-visible spectroscopy, then counting the velocity of drug release. The antimicrobial activity in vitro were examined by improved Kirby-Bauer diffusion method.Cells and scaffold of the morphology, adhesion and growth were observed by SEM. The average diameter of PLGA nanofibers with different tet contents between 360nm and 470nm . The 10% drug loaded membranes exhibited the highest initial burst release. Tet release sustained over 27 days in vitro. The antimicrobial activity in vitro was effective in inhibiting Staphylococcus aureus growth. Examination of the biocompatibility of PLGA/tet scaffolds with MG-63 cells showed no adverse effect on the cell adhesion and proliferation. So PLGA/tet nanofibers has good biocompatibility.

中图分类号: 

  • TS102.6
[1] 季涛, 李杰, 高强, 余进, 刘其霞. 粘胶基炭化织物的制备及工艺优化[J]. 纺织学报, 2012, 33(4): 49-54.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!