再生丝素蛋白/脱细胞真皮基质共混纳米纤维膜的制备及其性能

doi:10.13475/j.fzxb.20180705106

纺织学报 ›› 2019, Vol. 40 ›› Issue (07): 13-18.doi: 10.13475/j.fzxb.20180705106

再生丝素蛋白/脱细胞真皮基质共混纳米纤维膜的制备及其性能

林永佳¹, 杨董超², 张佩华¹(), 顾岩²

^1. 东华大学纺织面料技术教育部重点实验室, 上海 201620
^2. 上海交通大学医学院附属第九人民医院, 上海 200011

收稿日期:2018-07-20 修回日期:2019-04-10 出版日期:2019-07-15 发布日期:2019-07-25
通讯作者: 张佩华
作者简介:林永佳(1994-),女,硕士生。主要研究方向为生物医用纺织材料。
基金资助:
国家重点研发计划项目(2016YFB0303303);高等学校学科创新引智计划项目(B07024)

Preparation and properties of regenerated silk fibroin/acellular dermal matrix blended nanofiber membrane

LIN Yongjia¹, YANG Dongchao², ZHANG Peihua¹(), GU Yan²

^1. Key Laboratory of Textile Science & Technology of Ministry of Education, Donghua University, Shanghai 201620, China;
^2. Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Received:2018-07-20 Revised:2019-04-10 Online:2019-07-15 Published:2019-07-25
Contact: ZHANG Peihua

摘要/Abstract

摘要：

为进一步提升再生丝素蛋白(RSF)在生物组织工程中的应用潜力,将RSF与脱细胞真皮基质(ADM)按照不同质量比溶于甲酸制成纺丝液进行静电纺丝。借助台式扫描电子显微镜、傅里叶变换红外光谱仪、差式扫描量热仪及CCK8试剂盒对纳米纤维的形貌、微细结构及生物相容性进行研究。结果表明:在固定RSF与ADM质量比为9∶1、纺丝液质量浓度为13 g/mL时,所纺制纳米纤维形态更规整,纺丝状态更稳定;当纺丝液质量浓度为13 g/mL时,随着纺丝液中ADM占比的提高,纺丝液的黏度逐渐上升,可纺性变差; ADM与RSF之间存在相互作用,使共混纳米纤维膜中的部分无规结构逐渐向β折叠结构转变; RSF/ADM共混纳米纤维膜具有良好的生物相容性。

关键词: 生物医用材料, 静电纺丝, 再生丝素蛋白, 脱细胞真皮基质, 生物相容性

Abstract:

In order to further improve the application potential of regenerated silk fibroin (RSF) in biological tissue engineering, RSF and acellular dermal matrix (ADM) were dissolved in formic acid in different mass ratios to prepare a spinning solution for electrospinning. The morphology, microstructure and biocompatibility of nanofibers were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry and CCK8 kit. The test results show that at a fixed mass ratio of 9∶1, the nanofibers spun at a mass concentration of 13 g/mL are more regular, and the spinning state is more stable. When the fixed spinning solution mass concentration is 13 g/mL, as the proportion of ADM in the blend increases, the viscosity of the spinning solution increases gradually, the spinnability deteriorates, and the fiber morphology gradually becomes irregular. An interaction exists between ADM and RSF. Some of the random structures in the blended nanofiber membrane gradually shift to the β-sheet structure. The RSF/ADM blended nanofiber membrane has good biocompatibility.

Key words: biomedical material, electrospinning, regenerated silk fibroin, acellular dermal matrix, biocompatibility

中图分类号:

TS101.4

林永佳, 杨董超, 张佩华, 顾岩. 再生丝素蛋白/脱细胞真皮基质共混纳米纤维膜的制备及其性能[J]. 纺织学报, 2019, 40(07): 13-18.

LIN Yongjia, YANG Dongchao, ZHANG Peihua, GU Yan. Preparation and properties of regenerated silk fibroin/acellular dermal matrix blended nanofiber membrane[J]. Journal of Textile Research, 2019, 40(07): 13-18.

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质量浓度/(g·mL^-1)	平均直径/nm	CV值/%
9	358	41.6
11	462	24.5
13	616	30.4
15	685	35.6

RSF与ADM质量比	平均直径/nm	CV值/%
10∶0	618	19.0
9∶1	616	30.4
8∶2	507	38.1
7∶3	498	36.3
6∶4	499	24.8
5∶5	-	-

时间/d	OD值		P值
时间/d	共混纳米纤维膜	空白	P值
1	0.771±0.083	0.859±0.031	0.135
3	1.660±0.023	1.311±0.045	<0.01
5	2.644±0.059	2.552±0.028	0.051
7	2.823±0.052	2.829±0.039	0.878

再生丝素蛋白/脱细胞真皮基质共混纳米纤维膜的制备及其性能

Preparation and properties of regenerated silk fibroin/acellular dermal matrix blended nanofiber membrane

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