蚕丝蛋白/明胶复合水凝胶的结构与生物相容性

doi:10.13475/j.fzxb.20200301007

纺织学报 ›› 2020, Vol. 41 ›› Issue (11): 41-47.doi: 10.13475/j.fzxb.20200301007

蚕丝蛋白/明胶复合水凝胶的结构与生物相容性

王曙东¹^,²^,³, 马倩¹, 王可¹^,⁴, 瞿才新¹(), 戚玉²

1.盐城工业职业技术学院纺织服装学院, 江苏盐城 224005
2.苏州大学纺织与服装工程学院, 江苏苏州 215002
3.江苏金麦穗新能源科技股份有限公司, 江苏盐城 224005
4.青岛大学纺织服装学院, 山东青岛 262127

收稿日期:2020-03-04 修回日期:2020-08-12 出版日期:2020-11-15 发布日期:2020-11-26
通讯作者: 瞿才新
作者简介:王曙东(1983—),男,副教授,博士。主要研究方向为生物医用材料。
基金资助:
江苏省自然科学基金面上项目(SBK2020020632);江苏高校青蓝工程培养对象项目(苏教师2018 No.12);江苏高校青蓝工程培养对象项目(苏教师2019 No.3);江苏省高校自然科学基金面上项目(18KJB540005);江苏省高校自然科学基金面上项目(19KJD54000);国家留学基金委资助项目(201908370222)

Structure and biocompatibility of silk fibroin/gelatin blended hydrogels

WANG Shudong¹^,²^,³, MA Qian¹, WANG Ke¹^,⁴, QU Caixin¹(), QI Yu²

1. School of Textile and Clothing, Yancheng Polytechnic College, Yancheng, Jiangsu 224005, China
2. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215002, China
3. Jiangsu Jinmaisui New Energy Technology Co., Ltd., Yancheng, Jiangsu 224005, China
4. College of Textile and Clothing, Qingdao University, Qingdao, Shandong 262127, China

Received:2020-03-04 Revised:2020-08-12 Online:2020-11-15 Published:2020-11-26
Contact: QU Caixin

摘要/Abstract

摘要：

针对蚕丝蛋白水凝胶不易快速凝胶成型的问题,将明胶蛋白水溶液与蚕丝蛋白以一定质量比复合,通过挤出式3D打印设备制备得到蚕丝蛋白/明胶复合水凝胶,并对复合水凝胶的流变性能、微观结构和生物相容性进行分析。结果表明:明胶的添加提高了复合水凝胶的黏度和储能模量,有利于3D打印过程中蚕丝蛋白的快速凝胶成型;明胶的添加未对蚕丝蛋白的二级结构产生影响,通过3D打印法可制备具有微周期格栅状蚕丝蛋白/明胶复合水凝胶支架,该支架具有三维多孔结构;当蚕丝蛋白与明胶质量比为50∶50时,复合水凝胶支架的断裂强度达3.43 MPa,是纯蚕丝蛋白的3.9倍;培养7 d后,MC3T3-E1细胞可在复合水凝胶支架上生长、增殖和分化。

关键词: 蚕丝蛋白, 明胶, 复合水凝胶, 3D打印, 生物相容性, 支架材料, 生物医用材料

Abstract:

To address the difficulty in silk fibroin curing, gelatin and silk fibroin aqueous solution were blended, and the silk fibroin/gelatin blended hydrogels were prepared by three-dimensional printing. The rheological properties, microstructure and biological properties of the blended hydrogels were investigated. The results show that addition of gelatin increases the viscosity and energy storage modulus of blended hydrogels, which is good for the gel forming in the subsequent three-dimensional printing process. Addition of gelatin does not affect the secondary structure of silk fibroin, and the microperiodic silk fibroin/gelatin blended hydrogels were prepared by three-dimensional printing. The breaking strength of the blended scaffold (blending ratio of silk protein to gelatin was 50∶50) is 3.43 MPa, which is 3.9 times higher than that of the pure silk protein. The printed scaffold has a three-dimensional porous structure after freeze-drying. After 7 days' culturing, MC3T3-E1 cells were found to be able to grow, proliferate and differentiate on the blended hydrogel scaffolds.

Key words: silk fibroin, gelatin, composite hydrogel, three-dimensional printing, biocompatibility, scaffold material, biomedical material

中图分类号:

TS102.512

王曙东, 马倩, 王可, 瞿才新, 戚玉. 蚕丝蛋白/明胶复合水凝胶的结构与生物相容性[J]. 纺织学报, 2020, 41(11): 41-47.

WANG Shudong, MA Qian, WANG Ke, QU Caixin, QI Yu. Structure and biocompatibility of silk fibroin/gelatin blended hydrogels[J]. Journal of Textile Research, 2020, 41(11): 41-47.

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培养时间/d	吸光度
培养时间/d	蚕丝蛋白支架	蚕丝蛋白/明胶复合水凝胶支架
1	0.130±0.027	0.153±0.014
3	0.172±0.031	0.246±0.009
7	0.250±0.019	0.336±0.013

蚕丝蛋白/明胶复合水凝胶的结构与生物相容性

Structure and biocompatibility of silk fibroin/gelatin blended hydrogels

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