纺织学报 ›› 2022, Vol. 43 ›› Issue (05): 1-6.doi: 10.13475/j.fzxb.20211109306

• 特约专栏:第十一届中国纺织学术年会专家观点 •    下一篇

具有细胞黏附反差特性的单层平行丝素蛋白纤维图案的制备及其性能

顾张弘1,2, 姚响1,2, 王锦思1,2, 张耀鹏1,2()   

  1. 1.东华大学 纤维材料改性国家重点实验室, 上海 201620
    2.东华大学 材料科学与工程学院, 上海 201620
  • 收稿日期:2021-11-23 修回日期:2022-02-17 出版日期:2022-05-15 发布日期:2022-05-30
  • 通讯作者: 张耀鹏
  • 作者简介:顾张弘(1997—),男,硕士生。主要研究方向为生物纤维材料。
  • 基金资助:
    上海市自然科学基金项目(20ZR1402400);中央高校基本科研业务费专项资金资助项目(2232020D-04);上海市优秀学术带头人计划资助项目(20XD1400100);国家自然科学基金项目(52173031)

Preparation and properties of single-layer and parallel silk fibroin fiber patterns with cell adhesion contrast properties

GU Zhanghong1,2, YAO Xiang1,2, WANG Jinsi1,2, ZHANG Yaopeng1,2()   

  1. 1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China
    2. College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
  • Received:2021-11-23 Revised:2022-02-17 Published:2022-05-15 Online:2022-05-30
  • Contact: ZHANG Yaopeng

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摘要：

针对在分析纤维自身特征对细胞行为的影响时各类背景黏附产生干扰的问题,首先将寡聚乙二醇硅烷化试剂接枝在玻片表面得到具有抗细胞黏附特征的基底材料,然后利用湿法纺丝技术在基底表面制备可细胞黏附的单层丝素蛋白(SF)平行纤维,最后利用聚二甲基硅氧烷将SF纤维两端固定于基底上制备得到单层平行纤维图案,分析了纤维图案中纤维的直径、纤维间距、表面形貌和结构组成等特性,并以L929成纤维细胞为例检验该单层平行纤维图案的细胞黏附反差功能。结果表明:单层平行纤维图案中不同直径的SF纤维具有类似的外观形貌和二级结构组成特征,L929成纤维细胞仅可黏附在纤维表面,而不会黏附到基底背景区域,展现出优秀的细胞黏附反差特性。

关键词: 丝素蛋白纤维, 单层平行纤维图案, 抗细胞黏附, 纤维直径

Abstract:

Aiming at the interference of various background adhesion when investigating the influence of fiber characteristics on cell behavior, a substrate with anti-cell adhesion property was obtained by grafting of oligo-ethylene glycol silylation reagent on the surface of glass. The parallel single-layer silk fibroin (SF) fibers with cell adhesion property were prepared using wet spinning technology on the surface of the substrate, and the two ends of SF fibers were fixed on modified substrate by polydimethylsiloxane to prepare single-layer and parallel arranged fiber patterns. The diameter, surface morphology, structural component of SF fibers and fiber spacing in the constructed fiber patterns were characterized, and L929 was taken as an example to verify corresponding cell adhesion contrast properties of the constructed fiber patterns. Results show that the SF fibers with different diameters present similar morphological features and secondary structure contents. After cell seeding, the cells can only adhere to the surface of SF fibers, but not to the background substrate, confirming excellent cell adhesion contrast properties of the fiber patterns.

Key words: silk fibroin fiber, single-layer and parallel fiber patterns, anti-cell adhesion, fiber diameter

中图分类号: 

  • TS341.5

图1

具有细胞黏附反差特性的单层平行纤维图案组成示意图"

图2

细胞在MPTES修饰接枝前后玻片基底表面的黏附情况"

图3

2种不同直径单层平行纤维图案的相差显微图像"

表1

2种单层平行纤维图案中的纤维直径及纤维间间距统计结果"

纤维图案类别 直径/μm 纤维间间距/μm
S-SF纤维图案 83.1±7.7 194.2±23.2
L-SF纤维图案 128.6±11.6 159.9±20.7

图4

不同直径单层平行纤维图案中SF纤维的SEM照片"

图5

不同直径单层平行纤维图案中SF纤维的红外光谱图和二级结构含量统计结果 注:“△”表示单因素ANOVA分析中的概率值 p>0.05,无显著差异。"

图6

L929成纤维细胞在基底未修饰处理和修饰处理后的单层平行纤维图案上的黏附情况"

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