Abstract: Temperature and relative humidity are two important parameters in research of thermal and moisture comfort properties of clothes,and making accurate and real-time measuring of them is a practical technology.This article introduces the temperature and humidity measuring instrument which is developed on NI virtual instrument technology and LabVIEW software.This instrument has many functions,such as multi-channel signals input,real-time data acquiring and processing,graphical display,file preservation,and overrun alarming.This instrument can be used in thermal-moisture comfort measurement of clothes and some other relative fields,which is flexible,versatile and obtains good results in experiments.