Abstract:

In view of the drug-resistant characteristic of nosocomial bacterial infection, a novel photosensitive antimicrobial material pripared by grafting porphyrin were synthesized. Oxidized bacterial cellulose (BC-CHO) was prepared by immersing in sodium periodate (NaIO4) firstly, then ethylenediamine (EDA) was grafted onto the bacterial cellulose by reductive amination reaction, and finally protoporphyrin IX (PpIX) grafted ethylenediamined bacterial cellulose fibrous membrane was obtained by covalenting activated PpIX onto as-obtained ethylenediamined bacteria cellulose. Fourier transform infrared spectrometer, thermogravimetric analyzer, X-ray diffraction and scanning electron microscopy were utilized to analyze the functional groups, thermal properties, crystal structure of as-grafted fibrous membranes. At last, antibacterial behavior of BC-EDA-PpIX fibrous membrane and its reusability were discussed. The results show that amino spacer arm as well as the the a PpIX is successfully grafted onto the dialdehyde bacterial cellulose. Furthermore, the aldehyde content of BC-CHO gradually increased with the increase of NaIO4 concentration. Meanwhile, the thermal decomposition temperature decreased gradually, and the crystallinity slightly decreases. The first-timed antibacterial effect of the as-synthesized BC-EDA-PpIX fibrous membrane againse Staphylococcus aureus reached 98%, and such material still shows good antibacterial activity even after 5 times of ethanol washing.

Key words: photodynamic antibacterial material, bacterial cellulose, protoporphyrin IX, antibacterial property