Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (04): 64-71.doi: 10.13475/j.fzxb.20190700708

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Blue light initiated photocrosslinking of silk fibroin hydrogel

SUN Guangdong1, HUANG Yi1(), SHAO Jianzhong1, FAN Qinguo2   

  1. 1. Engineering Research Center for Eco-Dyeing and Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
    2. Department of Bioengineering,University of Massachusetts Dartmouth, North Dartmouth, Massachusetts 02747, USA
  • Received:2019-07-01 Revised:2019-11-26 Online:2020-04-15 Published:2020-04-27
  • Contact: HUANG Yi E-mail:yihuang@zstu.edu.cn

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Abstract:

A rapid fabrication method for high-strength silk fibroin hydrogel using blue light photocrosslinking was developed. To achieve this, different photoinitiators (camphorquinone, riboflavin sodium phosphate, curcumin and Eosin Y) together with diphenyl iodonium hexafluorophosphate (DPI) were composed to five efficient blue light photoinitiation systems for the photo-crosslinking of silk fibroin. The spectral absorption characteristics and the photopolymerization efficiency of five photoinitiators were investigated by UV-vis spectrophotometer and photo-DSC. The crosslinking performance of silk fibroin hydrogel was investigated by photo-rheology. The UV-vis spectrum indicates five photoinitiators can be served as blue light photoinitiators and photocrosslinking of silk fibroin can be attributed to the dityrosine by coupling of tyrosine residues. Camphorquinone and Eosin Y show a higher photopolymerization efficiency than other photoinitiators. With such photoinitiation systems, silk fibroin hydrogel is prepared within 10 min in this research.

Key words: silk fibroin hydrogel, photocrosslinking mechanism, photoinitiator, photosensitive synergist, dityrosine

CLC Number: 

  • O636.9

Fig.1

Spectral characteristics of four blue light. (a) UV-Vis absorption spectra of FMN, CC and EY in ethanol;(b) UV-Vis absorption spectra of CQ in ethanol"

Fig.2

Photosensitivity of blue light. (a) UV-Vis absorption spectra of camphorquinone under different blue light irradiation time; (b) Blue light bleaching properties of four photoinitiators"

Fig.3

Blue photopolymerization efficiency of AM initiated by different photoinitiation systems. (a) Heat flow of AM photopolymerized by different photoinitiators; (b) Double bond conversion of AM photopolymerized by different photoinitiators; (c) Heat flow of AM photopolymerized by photoinitiator/DPI system; (d) Double bond conversion of AM photopolymerized by photoinitiator/DPI system"

Fig.4

Photoinitiation mechanism of CQ/DPI initiation system"

Fig.5

UV-Vis spectra of EY photocrosslinked silk fibroin"

Fig.6

Photocrosslinking reaction and mechanism for silk fibroin"

Fig.7

Storage modulus, gel time and loss factor of photocrosslinked fibroin hydrogels under different photoinitiator systems. (a) Storage modulus without DPI; (b) Loss factor without DPI; (c) Storage modulus with DPI; (d) Loss factor with DPI; (e) Gel time"

Fig.8

Preparation process of photocrosslinked silk fibroin hydrogel. (a) Silk fibroin precursor before illumination;(b) Without DPI by illuminating blue light for 40 min; (c) With DPI by illuminating blue light for 10 min;(d) Silk fibroin hydrogel photoinitiated by photoinitiators for 10 min"

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