Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (09): 162-166.doi: 10.13475/j.fzxb.20200202205

• Column: Biomedical Textile Materials and It′s Products • Previous Articles     Next Articles

Preparation and in vitro inflammation evaluation of polydopamine coated polypropylene hernia mesh

QIAO Yansha1,2, WANG Qian1,2, LI Yan1,2,3, SANG Jiawen1, WANG Lu1,2,3()   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology of Ministry of Education, Donghua University, Shanghai 201620, China
    3. Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
  • Received:2020-02-12 Revised:2020-05-29 Online:2020-09-15 Published:2020-09-25
  • Contact: WANG Lu E-mail:wanglu@dhu.edu.cn

RichHTML

10

PDF (PC)

125

Abstract:

To reduce the possibility of inflammation with the use of polypropylene (PP) hernia mesh, dopamine was oxidized and self-polymerized for the preparation of an anti-inflammatory polydopamine (PDA) coating on the surface of PP monofilament. The micro-morphology, surface composition, water contact angle, coating stability, mechanical property, in vitro anti-oxidant and anti-inflammatory properties of PDA-PP were characterized and analyzed. The results show that PDA formed a coating of micro-nano particles with uniform distribution on PP fiber surfaces, and the coating changed physicochemical properties of PP mesh. Meanwhile, the original mechanical property of PP mesh is not affected by the PDA coating, and the antioxidant ability and good stability of the PDA coated PP mesh meet the needs of the initial inflammatory regulation. The protein expression level of the inflammation marker TNF-α and IL-6 at 24 h indicate a 93% and 100% lower expression levels for macrophages adhered PDA-PP mesh than that of the PP mesh, respectively. The PDA-PP mesh is expected for clinical evaluation studies next.

Key words: medical textiles, polypropylene mesh, inflammation, polydopamine, anti-oxidant, anti-inflammation

CLC Number: 

  • TS181.8

Fig.1

Apparent morphology of PP mesh (×10)"

Fig.2

SEM images of PP (a) and PDA-PP(b) (×3 000)"

Fig.3

FT-IR spectra of PP and PDA-PP"

Fig.4

XPS spectra of PDA-PP"

Fig.5

SEM images of PDA-PP at 1 d (a) and 3 d(b)"

Tab.1

Mechanical properties of PP and PDA-PP"

样品 断裂强度/(N·cm-1) 伸长率/%
横向 纵向 横向 纵向
PP 62.2±1.3 40.8±0.6 28.2±0.5 41.5±1.7
PDA-PP 63.6±2.1 41.6±0.8 27.2±1.1 42.0±0.6

Tab.2

CCK-8 absorbance value of L929 at 24 h"

样品 CCK-8吸光度值
PP 0.61±0.05
PDA-PP 0.55±0.04
空白对照样本 0.57±0.06

Tab.3

Concentration of TNF-α and IL-6 released from RAW 264.7"

样品 质量浓度/(pg·mL-1)
TNF-α IL-6
PP 189.13±2.13 4.8±0.6
PDA-PP 12.781±4.15 0
[1] JENKINS John T, O'DWYER Patrick J. Inguinal hernias[J]. British Medical Journal, 2008,336(7638):269-272.
doi: 10.1136/bmj.39450.428275.AD pmid: 18244999
[2] RUTKOW Ira M. Demographic and socioeconomic aspects of hernia repair in the United States in 2003[J]. Surgical Clinics of North America, 2003,83(5):1045-1051.
doi: 10.1016/S0039-6109(03)00132-4
[3] FRÄNNEBY U, SANDBLOM G, NORDIN P, et al. Risk factors for long-term pain after hernia surgery[J]. Annals of Surgery, 2006,244(2):212-219.
pmid: 16858183
[4] ANDRESEN K, ROSENBERG J. Management of chronic pain after hernia repair[J]. Journal of Pain Research, 2018,11:675-681.
pmid: 29670394
[5] 张方捷, 高国栋, 叶静, 等. 脂肪干细胞覆膜聚丙烯补片减轻疝修补术后炎症反应的研究[J]. 浙江中西医结合杂志, 2017 (3):180-183.
ZHANG Fangjie, GAO Guodong, YE Jing, et al. Effect of adipose-derived stem cells coated polypropylene patch on the inflammation after herniorrhaphy[J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2017 (3):180-183.
[6] WOLF M T, DEARTH C L, RANALLO C A, et al. Macrophage polarization in response to ECM coated polypropylene mesh[J]. Biomaterials, 2014,35(25):6838-6849.
pmid: 24856104
[7] LO T S, LIN Y H, YUSOFF F M, et al. The immunohistochemical and urodynamic evaluation towards the collagen-coated and non-coated polypropylene meshes implanted in the pelvic wall of the rats[J]. Scientific Reports, 2016,6(1):1-9.
doi: 10.1038/s41598-016-0001-8 pmid: 28442746
[8] BELEBECHA V, CASAGRANDE R, URBANO M R, et al. Effect of the platelet-rich plasma covering of polypropylene mesh on oxidative stress, inflammation, and adhesions[J]. International Urogynecology Journal, 2020,31(1):139-147.
pmid: 31129689
[9] LIU M Y, ZENG G J, WANG K, et al. Recent developments in polydopamine: an emerging soft matter for surface modification and biomedical applications[J]. Nanoscale, 2016,8(38):16819-16840.
pmid: 27704068
[10] RYU J H, MESSERSMITH P B, LEE H. Polydopamine surface chemistry: a decade of discovery[J]. ACS Applied Materials & Interfaces, 2018,10(9):7523-7540
doi: 10.1021/acsami.7b19865 pmid: 29465221
[11] ZHAO H, ZENG Z, LIU L, et al. Polydopamine nanoparticles for the treatment of acute inflammation-induced injury[J]. Nanoscale, 2018,10(15):6981-6991.
doi: 10.1039/c8nr00838h pmid: 29610822
[12] ZANGMEISTER R A, MORRIS T A, TARLOV M J. Characterization of polydopamine thin films deposited at short times by autoxidation of dopamine[J]. Langmuir, 2013,29(27):8619-8628.
pmid: 23750451
[13] JAMES M A. Biological responses to materials[J]. Annual Review of Materials Research, 2001,31:81-110.
doi: 10.1146/annurev.matsci.31.1.81
[14] YANG S S, WANG Y, WU X X, et al. Multifunctional tannic acid (TA) and lysozyme (Lys) films built layer by layer for potential application on implant coating[J]. ACS Biomaterials Science & Engineering, 2019,5(7):3582-3594.
pmid: 33405740
[15] ALZOGHAIBI M A. Concepts of oxidative stress and antioxidant defense in Crohn's disease[J]. World Journal of Gastroenterology, 2013,19(39):6540-6547.
pmid: 24151379
[16] REIMUND J M, HIRTH C, KOEHL C, et al. Antioxidant and immune status in active Crohn's disease: a possible relationship[J]. Clinical Nutrition, 2000,19(1):43-48.
doi: 10.1054/clnu.1999.0073 pmid: 10700533
[1] YANG Gang, LI Haidi, QIAO Yansha, LI Yan, WANG Lu, HE Hongbing. Preparation and characterization of polylactic acid-caprolactone / fibrinogen nanofiber based hernia mesh [J]. Journal of Textile Research, 2021, 42(01): 40-45.
[2] YANG Yuchen, QIN Xiaohong, YU Jianyong. Research progress of transforming electrospun nanofibers into functional yarns [J]. Journal of Textile Research, 2021, 42(01): 1-9.
[3] ZHANG Qian, MAO Jifu, LÜ Luyao, XU Zhongmian, WANG Lu. Abrasion resistance of suture at anchor eyelet for tendon-bone repair and its influencing factors [J]. Journal of Textile Research, 2020, 41(12): 66-72.
[4] ZHANG Xing, LIU Jinxin, ZHANG Haifeng, WANG Yuxiao, JIN Xiangyu. Preparation technology and research status of nonwoven filtration materials for individual protective masks [J]. Journal of Textile Research, 2020, 41(03): 168-174.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备14006648号
Copyright 2021 © Editorial office of Journal of Textile Research
Supported by Beijing Magtech Co.Ltd