Journal of Textile Research ›› 2025, Vol. 46 ›› Issue (01): 130-137.doi: 10.13475/j.fzxb.20231206001

• Dyeing and Finishing Engineering • Previous Articles     Next Articles

Degreasing finishing of cotton fabrics based on lipase immobilization and its application

CHAO Tanyu, YE Yun, LI Na, LIAO Sihan, MA Qikai, CUI Li()   

  1. College of Textile Science and Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China
  • Received:2023-12-29 Revised:2024-09-17 Online:2025-01-15 Published:2025-01-15
  • Contact: CUI Li E-mail:licui@jiangnan.edu.cn

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Abstract:

Objective Lipase is an important class of hydrolytic enzyme, and the hydrolysis substrates of lipase are usually natural oils and fats, where the hydrolysis site is the fatty acid and glycerol linked ester bond in oils and fats. Immobilized lipase has high stability and can be recycled for high quality products, having better properties than free lipase. Textile materials are a valid carrier for immobilized lipase, leading to special functional properties of the textiles.

Method The lipase molecule carries amino groups, and the hydroxyl groups of cotton can be oxidized into aldehyde groups selectively with sodium periodate. Therefore, the lipase molecules can be immobilized on the surface of cotton by the Schiff base reaction. Based on the above reaction principle, the oxidized cotton was used as the carrier for the immobilization of lipase to prepare textiles with degreasing function based on the hydrolysis of lipase. The immobilization conditions of lipase were investigated, and the enzymic properties of the immobilized lipase and the degreasing properties of the lipase-containing cotton fabric were investigated.

Results The suitable process conditions for the immobilization of lipase on cotton fabrics oxidized with sodium periodate were described as follows. 0.5 g cotton fabric was pretreated in 20 mL of 0.2 mol/L sodium periodate solution at 40 ℃ for 2 h. After sufficient washing, the lipase was cross-linked on cotton in 20 mL enzyme solution with pH 5.0 containing 15 g/L enzyme for 12 h at 4 ℃. The optimum temperature of the immobilized lipase was 50 ℃, which was similar to that of free lipase. Experiment results indicated that the immobilization process did not significantly affect the optimum temperature of lipase. However, the acid-resistant stability and temperature-resistant stability of immobilized lipase were improved compared with those of free lipase. Both the immobilized enzyme and free enzyme had similar stability under weak acidic conditions. While the stability of immobilized lipase was slightly better than that of free lipase under neutral conditions and the stability of immobilized lipase was significantly better than that of free lipase under strong acidic conditions. The immobilized lipase was significantly stabler than the free lipase at high temperatures. The immobilized lipase retained more than 50% of the activity when it was treated at 60 ℃ for 5 h, whereas the free enzyme activity decreased 80% of the activity. The immobilized lipase had certain operational stability and storage stability. When the cycles of operations exceeded 5, the enzyme activity residual rate of the immobilized enzyme began to decrease significantly, showing that the immobilized lipase had a better stability of operation. The activity of the immobilized lipase was decreased slowly when it was stored at 20 ℃ during the first week. After one week, enzyme activity was decreased rapidly. More than 50% activity of the lipase was lost after storing for 15 d. The immobilized lipase exhibited good degreasing activity for olive oil and edible chili oil. The oil stains on the cotton fabrics with immobilized lipase almost completely disappeared after the treatment in water, whereas a small amount of oil stains remained on the cotton fabrics without immobilized lipase. Fatty acid production was detected in the degreasing process of olive oil.

Conclusion The optimal pretreatment conditions for cotton fabric were found to be NaIO4 concentration of 0.20 mol/L and oxidation time of 2 h. The suitable conditions for lipase immobilization were identified to be lipase concentration of 15 g/L, reaction time of 12 h, pH value of 5.0, and reaction temperature of 4 ℃. The highest activity of the immobilized lipase was 35 U/g cotton fiber. Compared with the free lipase, the temperature stability and pH stability of the immobilized lipase were improved, and the lipase had better reusability and the cotton fabrics were endowed with good degreasing performance.

Key words: lipase, immobilization, cotton fabric, degreasing, enzyme stability, enzyme activity, degreasing finishing

CLC Number: 

  • TS195.5

Fig.1

Influence of pretreatment time on activity of immobilized lipases"

Fig.2

Influence of immobilization time on activity of immobilized lipase"

Fig.3

Influence of enzyme mass concentration on activity of immobilized lipase"

Fig.4

Influence of pH value on activity of immobilized lipase"

Tab.1

Influence of immobilization temperature on activity of immobilized lipase"

固定化温度/℃ 酶活力/(U·g-1)
4 29.63±0.67
20 19.84±0.56
40 15.12±0.39

Fig.5

Optimum temperature of lipase"

Fig.6

Temperature stability of lipase"

Fig.7

pH value stability of lipase"

Fig.8

Reusable stability of immobilized lipase"

Fig.9

Storage stability of immobilized lipase"

Fig.10

Degreasing effect of immobilized lipase on olive oil. (a) Before immobilized lipase reaction; (b) After immobilized lipase reaction; (c) Before unimmobilized lipase reaction; (d) After unimmobilized lipase reaction"

Fig.11

Degreasing effect of immobilized lipase on chili oil. (a) Before immobilized lipase reaction; (b) After immobilized lipase reaction; (c) Before unimmobilized lipase reaction; (d) After unimmobilized lipase reaction"

Fig.12

Liquid-mass analysis results of oil phase extracted from cotton fabric and buffer residue. (a) Oil phase extracted from immobilized lipase cotton fabric; (b) Oil phase extracted from blank cotton fabric; (c) Phosphate buffer residue after reaction of immobilized lipase in cotton fabric; (d) Phosphate buffer residue after reaction of blank cotton fabric"

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