Abstract: According to the movement analysis and mathematical models of glove knitting mechanism, a analytical software was programmed by Visual Basic 6 0.This software has good human-computer interactions in collaboration. By the intuitive and logical thought of human and the target of optimization, many groups of parameters which could satisfy the quest of transplanting were found with the software. Orthogonal experiment had been made with four better levels of every parameter.A best group of parameters had been easily found by SAS, which proved that it is better than those of original by dynamic simulation and analysis.