Abstract: Using mixed seed liquid of bacillus B2 and aspergillus M2 for degumming of ramie, we can confirm the optimal conditions of joint strains enzymatic degumming process by the L18（36） orthogonal experiment:bacillus B2 and aspergillus M2 seed liquid inoculation amount of 6% and 11%, respectively, the proportion of water and material is 15:1 (mL/g),unglued initial pH value is 6, temperature for 35℃, and unglued time is 50 h.Under the best condition, unglued rate of treated ramie can reach 31.9%, fibre fineness and fibre breaking strength are accord with the standard of secondary ramie. Using the infrared spectrometry and scanning electron microscope for the test and analysis, the results proved that after unglued by biological enzyme,obtained the smooth, flat and slim ramie, of which the molecular structure of the glue complex changed obviously.

Key words: ramie , microorganism degumming , orthogonal experiment , fibrous structure