JOURNAL OF TEXTILE RESEARCH ›› 2014, Vol. 35 ›› Issue (11): 102-0.

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Isolation and identification of an actinomycete strain DM182 for microbial degumming of hemp

  

  • Received:2013-12-27 Revised:2014-05-18 Online:2014-11-15 Published:2014-11-20
  • Contact: Xue-jun WANG E-mail:xjwang0917@163.com

Abstract: The study was to explore whether the degumming strains exist in soil sample collected from the roots of hemp. The actinomycetes were cultivated on GAUZE’s Medium NO.1. The degumming strains of hemp were screened by the method of transparent zones. The strain was classified by physiological, biochemical and morphological characteristics and 16S rRNA gene sequence analysis. The results showed that a total of 41 actinomyctes were isolated from the samples, of which the strain DM182 was more effective in decomposing the pectin of hemp, and the transparent circle diameter is 3.78cm. According to its morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain DM182 was preliminary identified as Nocardioides albus. Thus, some members of this genus have a very wide application prospect in agriculture and industry. This is the first report that N. albus strain was isolated in the local region, and our study provided basis for the further exploration of the strain.

CLC Number: 

  • Q939.97、TS192155
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