Abstract: Introduced a new microbial transglutaminase(MTG) which was prepared by microbial fermentation and applied to the treatment of wool fabrics,and investigated its effect in anti-felting and remedy of strength loss of the wool fabric.Analysis of amino-acids of the wool used in this experiment was undertaken to provide a theoretic basis for the feasibility of the role of MTG.The wool fabric was pretreated in order by potassium permanganate and protease,then,followed by MTG treatment.When compared with the control sample,the strength of the treated fabric increased by about 30%,and the felting shrinkage reduced from 9.3% to 2.3%.When compared with the traditional resin treatment,its felting shrinkage was similar,but strength was lower.However,the COD of its treating bath was only one third of that of the traditional resin treatment.