纺织学报 ›› 2021, Vol. 42 ›› Issue (08): 90-95.doi: 10.13475/j.fzxb.20200808206

• 染整与化学品 • 上一篇    下一篇

黄麻纤维中黄酮类化合物提取工艺优化及表征

崔启璐1,2, 李佳蔚1, 潘柳桂1, 杨飞1,3, 郁崇文1,2()   

  1. 1.东华大学 纺织学院, 上海 201620
    2.东华大学 纺织面料技术教育部重点实验室, 上海 201620
    3.浙江金鹰股份有限公司, 浙江 舟山 316051
  • 收稿日期:2020-08-20 修回日期:2021-05-06 出版日期:2021-08-15 发布日期:2021-08-24
  • 通讯作者: 郁崇文
  • 作者简介:崔启璐(1988—),女,博士生。主要研究方向为麻纤维性能。
  • 基金资助:
    国家麻类产业技术体系项目(CARS-16)

Optimization and characterization of flavonoids extracted from jute fiber

CUI Qilu1,2, LI Jiawei1, PAN Liugui1, YANG Fei1,3, YU Chongwen1,2()   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China
    3. Zhejiang Golden Eagle Co., Ltd., Zhoushan, Zhejiang 316051, China
  • Received:2020-08-20 Revised:2021-05-06 Published:2021-08-15 Online:2021-08-24
  • Contact: YU Chongwen

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摘要：

为充分开发黄麻纤维的应用价值,探究其中是否含有黄酮类化合物,采用水醇提取法制备了黄麻纤维提取液,利用紫外分光光度法测试提取液中黄酮含量,按照二次通用旋转组合设计方法对提取工艺进行优化,研究了乙醇体积分数、浴比、提取时间3个工艺因子与黄酮类化合物提取率之间的关系。对经过旋转蒸发和真空冷冻干燥后黄麻纤维提取物的化学结构、热稳定性和表面形貌进行测试与分析。结果表明:黄麻纤维提取物与芦丁标准品化学结构相似,说明其中含有黄酮类化合物,其最优提取工艺是乙醇体积分数75%,浴比1∶50,提取时间150 min, 该条件下黄酮的提取率为0.037%。

关键词: 黄麻纤维, 黄酮, 响应面分析, 提取工艺, 水醇提取法

Abstract:

In order to confirm the existence of flavonoids in jute fiber and to develop the application value, the jute fiber extracts were obtained using alcohol-water method. The content of flavonoids in the extraction solution was tested using ultraviolet spectrophotometer. The extraction process was optimized based on the use of quadratic general rotary design, and the relationships between the three factors(alcohol volume fraction, bath ratio and extracting time) and the extraction rate of flavonoids were studied. The chemical structure, thermal stability and morphology of jute fiber extracts after rotary evaporation and vacuum freeze drying were tested and analyzed. The results show that the chemical structure of jute fiber extracts is similar to the structure of rutin standard, which indicates that it contains flavonoids. The optimal extraction conditions were found to be ethanol volume fraction 75%, bath ratio 1∶50, and extracting time 150 min, at which the extraction rate of flavonoids was 0.037%.

Key words: jute fiber, flavonoid, responsive surface analysis, extraction process, alcohol-water method

中图分类号: 

  • TS121

表1

试验因子水平编码表"

水平 A
乙醇体积分数x1/%		 B
浴比x2		 C
时间x3/min
-1.682(-γ) 60 1∶30 60
-1 64 1∶34 78
0 70 1∶40 105
1 76 1∶46 132
+1.682(+γ) 80 1∶50 150

表2

试验设计及黄酮类化合物提取率"

试验号 x0 A B C 提取率ye/%
1# 1 1 1 1 0.007 8
2# 1 1 1 -1 0.012 2
3# 1 1 -1 1 0.012 2
4# 1 1 -1 -1 0.014 7
5# 1 -1 1 1 0.013 3
6# 1 -1 1 -1 0.013 3
7# 1 -1 -1 1 0.007 4
8# 1 -1 -1 -1 0.018 3
9# 1 1.682 0 0 0.004 9
10# 1 -1.682 0 0 0.003 9
11# 1 0 1.682 0 0.006 1
12# 1 0 -1.682 0 0.018 6
13# 1 0 0 1.682 0.008 7
14# 1 0 0 -1.682 0.020 1
15# 1 0 0 0 0.012 5
16# 1 0 0 0 0.023 0
17# 1 0 0 0 0.014 4
18# 1 0 0 0 0.015 3
19# 1 0 0 0 0.007 7
20# 1 0 0 0 0.017 2

表3

回归系数求解及显著性检验结果"

项目 数值 t检验 显著性
b0 0.013 2.182 476>t0.1(10) 显著
b1 -0.003 654 0.924 61<t0.1(10) 不显著
b2 -0.002 69 0.680 67<t0.1(10) 不显著
b3 -0.003 974 1.005 57<t0.1(10) 不显著
b12 -0.002 708 1.658 43<t0.1(10) 不显著
b13 0.006 855 4.197 795>t0.01(10) 显著
b23 0.004 458 2.799 45>t0.02(10) 显著
b11 -0.008 6 2.235 4>t0.05(10) 显著
b22 -0.006 5 1.689 63<t0.1(10) 不显著
b33 0.002 561 0.665 714> t0.1(10) 显著

表4

回归方程的显著性检验结果"

指标 F1 F2 Fα1 Fα2 显著性
提取率 0.66 1.68 F0.01(5,5)=11.0 F 0 . 25(9,10)=1.56 显著

图1

不同工艺及其交互作用对黄酮提取率的影响"

表5

二次通用旋转试验验证结果"

试验号 因子 指标
y/%
x1/% x2 x3/min
8# 64 1∶34 78 0.018 3
12# 70 1∶30 105 0.018 6
14# 70 1∶40 60 0.020 1
16# 70 1∶40 105 0.023 0
最优解 预测值 75 1∶50 150 0.036 1
实测值 75 1∶50 150 0.037 0
较优解1 预测值 75 1∶47 150 0.032 3
实测值 75 1∶47 150 0.019 8
较优解2 预测值 75 1∶46 150 0.031 5
实测值 75 1∶46 150 0.018 0

图2

芦丁标准品和黄麻纤维提取物的红外光谱图"

图3

黄麻纤维提取物的表面扫描电镜照片(×300)"

表6

黄麻纤维提取物元素组成及含量"

元素 质量百分比/% 标准偏差
C 69.81 0.90
O 24.87 0.84
Na 0.43 0.11
Si 2.14 0.14
Cl 1.11 0.14
K 0.67 0.14
Ca 0.97 0.18

图4

黄麻纤维提取物的热性能"

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