纤维素结合域的引入对角质酶在纤维素类纤维上吸附性能的影响

纺织学报 ›› 2012, Vol. 33 ›› Issue (2): 55-58.

纤维素结合域的引入对角质酶在纤维素类纤维上吸附性能的影响

周丽华, 王强, 范雪荣, 吴敬, 陈昀, 曹建华, 刘喆

1. 江南大学
2. 南通醋酸纤维有限公司

收稿日期:2011-03-02 修回日期:2011-04-27 出版日期:2012-02-15 发布日期:2012-02-15
通讯作者: 王强 E-mail:qiang_wang@163.com
基金资助:
国家级;省级;校级

Influence of introduction of CBD on adsorption of cutinase on cellulose fibers

ZHOU Li-Hua, WANG Qiang, FAN Xue-Rong, WU Jing, CHEN Yun, CAO Jian-Hua, LIU Zhe

Received:2011-03-02 Revised:2011-04-27 Online:2012-02-15 Published:2012-02-15

摘要/Abstract

摘要： 为了研究纤维素结合域（Cellulose-binding Domain，CBD）的引入对角质酶在纤维素类纤维上吸附能力的影响，本文选用漂白棉织物、粘胶纤维、醋酯纤维以及纯棉针织坯布这四种材质，对角质酶与CBD-角质酶进行吸附对比试验。研究表明：CBD结构的引入，可提高角质酶在纤维素纤维上的吸附量。但是当纤维素葡萄糖残基上羟基被部分酯化后，CBD结构的引入对吸附量造成的影响开始减弱，而当纤维素纤维表面被疏水性杂质覆盖时，CBD-角质酶对纤维素纤维的吸附能力反而降低。

关键词: 角质酶, 纤维素结合域, 纤维素, 吸附性能

Abstract: To study the influence of adsorption capacity in the cellulose fiber when fusing Cellulose-binding Domain（CBD） into cutinase, we selected bleached cotton, viscose fiber, cellulose acetate fiber, and prey cotton knitted fabric, and had comparative tests about adsorption capacities between cutinase and cutinase-CBD. The results show that: after fusing CBD into the cutinase the binding protien amount between cutinase and cellulose fibers have inhanced. In case the glucose residue of cellulose hydroxyl groups are partially esterified, the impact of the introduction of CBD structure on the adsorption began to weaken. On condition the cellulose fiber surface is covered by the hydrophobic impurities, the adsorption capacity of cutinase-CBD decreased.

Key words: cutinase, cellulose-binding domain, cellulose, adsorption capacity

周丽华, 王强, 范雪荣, 吴敬, 陈昀, 曹建华, 刘喆. 纤维素结合域的引入对角质酶在纤维素类纤维上吸附性能的影响[J]. 纺织学报, 2012, 33(2): 55-58.

ZHOU Li-Hua, WANG Qiang, FAN Xue-Rong, WU Jing, CHEN Yun, CAO Jian-Hua, LIU Zhe. Influence of introduction of CBD on adsorption of cutinase on cellulose fibers[J]. JOURNAL OF TEXTILE RESEARCH, 2012, 33(2): 55-58.

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