阴离子交换型乙烯-乙烯醇共聚物纳米纤维气凝胶蛋白分离材料

doi:10.13475/j.fzxb.20240104901

纺织学报 ›› 2025, Vol. 46 ›› Issue (04): 29-37.doi: 10.13475/j.fzxb.20240104901

阴离子交换型乙烯-乙烯醇共聚物纳米纤维气凝胶蛋白分离材料

曹展瑞¹^,², 纪灿灿¹^,², 赫羴姗¹^,², 周丰¹^,³, 向阳³, 高飞³, 刘轲¹^,²(), 王栋¹^,²

1.武汉纺织大学纺织纤维及制品教育部重点实验室, 湖北武汉 430200
2.武汉纺织大学纺织行业非织造过滤与分离材料重点实验室, 湖北武汉 430200
3.安海斯布希企业管理(上海)有限公司武汉分公司, 湖北武汉 430051

收稿日期:2024-01-29 修回日期:2024-11-18 出版日期:2025-04-15 发布日期:2025-06-11
通讯作者: 刘轲(1984—),男,教授,博士。主要研究方向为纤维基过滤分离材料。E-mail:kliu@wtu.edu.cn。
作者简介:曹展瑞(1997—),男,硕士生。主要研究方向为纤维基梯度过滤分离材料。
基金资助:
国家自然科学基金项目(U23A20585);国家自然科学基金项目(52273061);湖北省自然科学基金杰出青年项目(2023AFA086);湖北省技术创新专项重大项目(2021BAB119);武汉市科技计划项目(2023030103010633)

Preparation and bovine serum albumin separation of ethylene vinyl alcohol copolymer nanofibrous anion-exchange aerogel

CAO Zhanrui¹^,², JI Cancan¹^,², HE Shanshan¹^,², ZHOU Feng¹^,³, XIANG Yang³, GAO Fei³, LIU Ke¹^,²(), WANG Dong¹^,²

1. Key Laboratory of Textile Fibers and Products, Ministry of Education, Wuhan Textile University, Wuhan, Hubei 430200, China
2. Key Laboratory of Nonwoven Filtration and Separation Materials for Textile Industry, Wuhan Textile University, Wuhan, Hubei 430200, China
3. Wuhan Branch, Anheuserbusch Enterprise Management (Shanghai) Co., Ltd., Wuhan, Hubei 430051, China

Received:2024-01-29 Revised:2024-11-18 Published:2025-04-15 Online:2025-06-11

摘要/Abstract

摘要： 针对传统微球层析材料对牛血清蛋白等生物制品的分离速度慢、分离效率低的问题,采用冷冻干燥方法制备出乙烯-乙烯醇共聚物(EVOH)纳米纤维-壳聚糖季铵盐(CS)复合气凝胶材料,并通过扫描电子显微镜、X射线光电子能谱仪、傅里叶红外光谱仪等对气凝胶吸附材料的表面形貌和表面化学组成进行了表征和分析。结果表明,该气凝胶材料具有以EVOH纳米纤维为骨架的互穿多孔结构,压缩回弹性优异,对牛血清白蛋白(BSA)的静态饱和吸附量高达1 121.6 mg/g。本研究为生物大分子分离和纯化材料的构筑提供了一种简单高效的方法。

关键词: 乙烯-乙烯醇共聚物纳米纤维, 壳聚糖季铵盐, 气凝胶, 牛血清白蛋白吸附

Abstract:

Objective Traditional microsphere chromatography materials have disadvantages in pontificating bovine serum albumin and other biological products with slow separation speed and low separation efficiency. To solve this issue, ethylene vinyl alcohol copolymer (EVOH) nanofibers and chitosan quaternary ammonium salt have been adopted to prepare composite aerogel materials by freeze-drying method. This study provides a simple and efficient method for the construction of biomacromolecule separation and purification materials.

Method EVOH nanofibers were used as support framework material, glutaraldehyde was used as crosslinking agent, and chitosan quaternary ammonium salt was used as modifier and binder to prepare aerogel materials. The surface morphology and surface chemical composition of the composite material were characterized and analyzed using SEM, XPS, FT-IR, and other characterization techniques. The mechanical properties of aerogel materials were characterized by universal tensile testing machine. The hydrophilicity of aerogel materials in dry and wet environment was characterized by contact angle tester. The adsorption capacity test was used to study the BSA adsorption performance of aerogels.

Result Aerogel materials possesses the laminated or spheric structure derived from EVOH nanofibers and chitosan quaternary ammonium salt solution blends in via freeze-drying process. For EVOH/CS-25%, spheric structure has been obtained different to that of other samples with more or less the content of chitosan quaternary ammonium salt.With the decrease of chitosan quaternary ammonium salt, the aerogel materials present decreased water contact angle and shorter time spent to total infusion of water. In addition, as the content of chitosan quaternary ammonium salts decreases, the water absorption of the materials also gradually decreases implying the combined function of chitosan derivative and nanofibrous scaffold. Through the infrared spectra and X-ray photoelectron spectra, it can be seen that aerogel materials have new absorption peaks at 1 645 cm^-1, and a wide peak at 3 325 cm^-1 different from EVOH nanofibers, which belongs toa vibration caused by the quaternary ammonium group of chitosan quaternary ammonium salts. These results suggest that chitosan quaternary ammonium salts are successfully crosslinked to the EVOH nanofiber surface. It is worth noting that when the content of chitosan quaternary ammonium salt is 17%, the adsorption performance is lower than that of the concentration of 25%, it can be seen that increasing the content of chitosan quaternary ammonium salt is conducive to improving the adsorption performance. Compared with other aerogels with chitosan quaternary ammonium salt content, EVOH/CS-25% has a through-pore structure and good structural stability, so that the saturated adsorption capacity is larger. These results indicate that the adsorption performance of aerogels is jointly affected by the content of chitosan quaternary ammonium salts and the porous structure of nanofiber scaffold.The unique structure of EVOH/CS-25% provides more continuous three-dimensional pores, which is beneficial to the improved mechanical performance to withstand pressure and decompression in air and water indicating a good compression resistance during ion exchange chromatography.

Conclusion A novel aerogel adsorbent was prepared by combining EVOH nanofibers with chitosan quaternary ammonium salts. The nanofiber aerogel materials present a unique microporous network structure and higher specific surface area than the membranes with the same chemical components. The results showed that the composite adsorbent effectively adsorbed the BSA, with a static saturation adsorption capacity of up to 1 121.6 mg/g and a shorter time for saturation adsorption, which can be assigned to the three-dimensional structure of aerogel material with higher specific surface area and interconnected porous channels. Therefore, this work provides a new and efficient approach for the development of anion exchange materials with fast adsorption rate and large adsorption capacity.

Key words: ethylene-vinyl alcohol nanofiber, chitosan quaternary ammonium salt, aerogel, bovine serum albumin

中图分类号:

TH145

曹展瑞, 纪灿灿, 赫羴姗, 周丰, 向阳, 高飞, 刘轲, 王栋. 阴离子交换型乙烯-乙烯醇共聚物纳米纤维气凝胶蛋白分离材料[J]. 纺织学报, 2025, 46(04): 29-37.

CAO Zhanrui, JI Cancan, HE Shanshan, ZHOU Feng, XIANG Yang, GAO Fei, LIU Ke, WANG Dong. Preparation and bovine serum albumin separation of ethylene vinyl alcohol copolymer nanofibrous anion-exchange aerogel[J]. Journal of Textile Research, 2025, 46(04): 29-37.

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链接本文: http://www.fzxb.org.cn/CN/10.13475/j.fzxb.20240104901

http://www.fzxb.org.cn/CN/Y2025/V46/I04/29

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阴离子交换型乙烯-乙烯醇共聚物纳米纤维气凝胶蛋白分离材料

Preparation and bovine serum albumin separation of ethylene vinyl alcohol copolymer nanofibrous anion-exchange aerogel

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