Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (05): 1-6.doi: 10.13475/j.fzxb.20211109306

• Invited Column: Expert Opinion of the 11th China Textile Academic Conferenc •     Next Articles

Preparation and properties of single-layer and parallel silk fibroin fiber patterns with cell adhesion contrast properties

GU Zhanghong1,2, YAO Xiang1,2, WANG Jinsi1,2, ZHANG Yaopeng1,2()   

  1. 1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China
    2. College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
  • Received:2021-11-23 Revised:2022-02-17 Online:2022-05-15 Published:2022-05-30
  • Contact: ZHANG Yaopeng E-mail:zyp@dhu.edu.cn

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Abstract:

Aiming at the interference of various background adhesion when investigating the influence of fiber characteristics on cell behavior, a substrate with anti-cell adhesion property was obtained by grafting of oligo-ethylene glycol silylation reagent on the surface of glass. The parallel single-layer silk fibroin (SF) fibers with cell adhesion property were prepared using wet spinning technology on the surface of the substrate, and the two ends of SF fibers were fixed on modified substrate by polydimethylsiloxane to prepare single-layer and parallel arranged fiber patterns. The diameter, surface morphology, structural component of SF fibers and fiber spacing in the constructed fiber patterns were characterized, and L929 was taken as an example to verify corresponding cell adhesion contrast properties of the constructed fiber patterns. Results show that the SF fibers with different diameters present similar morphological features and secondary structure contents. After cell seeding, the cells can only adhere to the surface of SF fibers, but not to the background substrate, confirming excellent cell adhesion contrast properties of the fiber patterns.

Key words: silk fibroin fiber, single-layer and parallel fiber patterns, anti-cell adhesion, fiber diameter

CLC Number: 

  • TS341.5

Fig.1

Schematic diagram to show component of single-layer and parallel arranged fiber pattern with cell adhesion contrast properties"

Fig.2

Cell adhesion on glass surfaces before (a) and after (b)MPTES grafting modification"

Fig.3

Phase contrast micrographs of single-layer and parallel arranged fiber patterns with different diameters"

Tab.1

Statistical results of fiber diameter and fiber spacing of single-layer and parallel arranged fiber patterns"

纤维图案类别 直径/μm 纤维间间距/μm
S-SF纤维图案 83.1±7.7 194.2±23.2
L-SF纤维图案 128.6±11.6 159.9±20.7

Fig.4

SEM images of SF fibers in single-layer and parallel arranged fiber patterns with different diameters"

Fig.5

FT-IR spectrum (a) and statistical results of secondary structure contents (b) of SF fibers in single-layer and parallel arranged fiber patterns"

Fig.6

Adhesion of L929 cells on single-layer and parallel arranged fiber patterns with unmodified or modified substrate. (a)Inverted phase contrast microscope images; (b)SEM images"

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