Abstract: The mechanical properties of spider dragline silk reeled to the different spiders to be anaesthetized were investigated.The dragline silk were reeled in different tension and humidity,and their mechanical properties were measured,which is useful to the artificial spinning of protein fiber.The results showed that the stress and diameter of dragline silk decrease with the anaesthetized time increasing,but the values of other mechanical characteristics don't apparently decrease.It could be concluded that the protein structure in spider's ampullate gland determine the properties of dragline silk when spiders lost their control ability.When the tension is 0\^039 cN,dragline silk has the best mechanical properties.The strain of dragline silk increase a little while the values of other mechanical characteristics decrease when the silk is reeled in wet.