Abstract: The technique for applying and fixing nano-material on cotton fabric was studied,and the UV protective effect and durability of the treated samples were investigated.The impact of process parameters on the results of application of nano-material of the fabric using fixing agents was discussed and process was optimized.The results show that 3~# fixing agent has excellent fixing effect.With optimized process conditions,UPF values of the finished cotton fabrics can reach the grade of "excellent"(Australian / New Zealand Standards) and maintain the grade of "very good" after 20 soaping.Tested according to AATCC standard method 100,the anti-bacteria effect reached 100% and maintained 98% after 20 soaping.