Abstract: For commercial application of Flos Sophorae buds dyes(FSBD),its chemical composition and resistance to heat,acid and alkali were tested,and the effects of dyeing processes such as temperature,pH value,the dosage(o.w.f) of aluminum mordant and FSBD on the result of the dyed wool fabric were investigated.The anti-microbial activity of the dyed wool fabric with FSBD was tested also.The experimental results showed: the FSBD powder,which has high purity and good stability properties to heat and acid,can be produced by the simple and low-cost method;the yellow wool fabric with brilliant shade and good color fastnesses can be obtained by the optimized pre-mordant dyeing process with aluminum;the dyed wool fabric has bactericidal property against staphylococcus aureus and inhibitory effect against bacillus coli and Candida albicans.