Abstract: To establish a method for the determination of five yellow disperse dyestuffs，such as Disperse Yellow 1，Disperse Yellow 3，Disperse Yellow 9，Disperse Yellow 39 and Disperse Yellow 49 in ecological textile by ultra-fast liquid chromatography coupled with tandem mass spectrometry (UFLC-MS/MS)．After the sample is extracted by methanol，the extract is separated on an ultra-fast liquid chromatography column by gradient elution with water and acetonitrile．Detection was carried out by UFLC-MS/MS using electrospray ionization interface in the multi-reaction monitoring (MRM) mode．Calibration curves were linear within the range of 1.0~100.0 μg/kg with the correlation coefficients of more than 0.999，and the limits of quantification （LOQs）were between 0.5~1.0 μg/kg．The extraction recoveries of the five disperse dyestuffs are at 84.5%~106.2%， and the RSDs were between 1.2%~6.4%.The developed method is 　quick，simple，sensitive and specificity for the satisfactory determination of disperse dyestuffs in ecological textile．

Key words: UFJC-MS/MS, ecological textile, disperse dyestuff, linear dependence