纺织学报 ›› 2019, Vol. 40 ›› Issue (02): 20-25.doi: 10.13475/j.fzxb.20181006406

• 纤维材料 • 上一篇    下一篇

紫外-可见分光光度法在定量监测2,3,5-三碘苯甲酸缓释中的应用

刘来俊1,2, 徐海燕1,2, 赵帆1,2, 王富军1,2(), 王璐1,2   

  1. 1.东华大学 纺织学院, 上海 201620
    2.东华大学 纺织面料技术教育部重点实验室, 上海 201620
  • 收稿日期:2018-10-31 修回日期:2018-11-16 出版日期:2019-02-15 发布日期:2019-02-01
  • 通讯作者: 王富军
  • 作者简介:刘来俊(1992—),男,博士生。主要研究方向为生物医用骨修复纺织材料。
  • 基金资助:
    高等学校学科创新引智计划资助项目(b07024);上海市科技支撑计划资助项目(16441903803);上海市科技支撑计划资助项目(18441902600)

Application of UV-Vis spectrophotometry in quantitative monitoring sustained release of 2,3,5-triiodobenzoic acid

LIU Laijun1,2, XU Haiyan1,2, ZHAO Fan1,2, WANG Fujun1,2(), WANG Lu1,2   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620,China
  • Received:2018-10-31 Revised:2018-11-16 Online:2019-02-15 Published:2019-02-01
  • Contact: WANG Fujun

摘要:

为研究显影剂2,3,5-三碘苯甲酸(TIBA)在显影纤维聚对二氧环己酮(PPDO)降解液中的缓释规律,采用紫外可见分光光度法探究了TIBA在不同体积比的磷酸盐缓冲溶液(PBS)和乙醇共混降解液(VPBS:V乙醇分别为1:3、1:1和3:1)中的紫外-可见分光光谱和回归曲线,根据回归曲线,测定降解液的吸光度值及计算PPDO纤维在降解过程中显影剂TIBA的释放量,借助药物缓释理论模型进行拟合,并分析相应的释放规律。结果表明:在3种共混溶液中,均能得到回归关系明显的线性方程,考虑到乙醇用量,选择PBS与乙醇的体积比为3:1的溶液作为标准液,并且溶液在37 ℃的环境中静置8 d后仍能保持一定的稳定性;模型拟合结果表明TIBA的释放规律符合一级释放的函数模型。

关键词: 紫外-可见分光光度法, 显影纤维, 纤维降解, 2,3,5-三碘苯甲酸, 释放模型

Abstract:

In order to study the sustained release rules of 2,3,5-triiodobenzoic acid (TIBA) in degradation solution of radiopaque poly-p-dioxanone (PPDO) filaments, the UV spectrums and regression curves of TIBA in different volume ratio blend degradation solutions of phosphate buffered saline (PBS) and ethanol (VPBS:Vethanol were 1:3,1:1 and 3:1) were explored by UV-Vis Spectrophotometry. According to regression curve, the release amount of TIBA in the degradation period was measured and calculated and then fitted with a drug release models and the corresponding release rules were analyzed. The results show that ideal spectrums and regression curves could be obtained in three kinds of blend solution. Considering the amount of ethanol used, the volume ratio of 3:1 was selected as the standard solution and it can still maintain stability after placing in an environment of 37 ℃ for 8 d. The results of model fit show that the release rule of TIBA is in consistence with the first-order release model.

Key words: ultraviolet-visible spectrophotometry, developing fiber, fiber degradation, 2,3,5-triiodobenzoic acid, release models

中图分类号: 

  • TS101

图1

降解液收集流程示意图"

图2

TIBA在R1溶液中的光谱图和回归分析"

图3

TIBA在R2溶液中的光谱图和回归分析"

图4

TIBA在R3溶液中的光谱图和回归分析"

图5

8 d后TIBA在R3溶液中的光谱图和回归分析"

表1

药物释放常规模型"

释放模型 函数模型 释放机制
零级释放 Mt/M0=kt 恒速释放
一级释放 ln(1-Mt/ M0)=-kt 一级释放
Peppas-Korsmeyer方程 Mt/M0=ktn 扩散和溶蚀
Higuchi方程 Mt/M0=kt1/2 Fick扩散

图6

S1、S2和S3 中TIBA的累积释放率"

表2

S1、S2和S3中TIBA缓释拟合参数"

释放模型 释放动力学系数k R2
S1 S2 S3 S1 S2 S3
零级释放 0.044 9 0.043 3 0.044 7 0.687 0.837 0.802
一级释放 0.506 2 0.168 1 0.216 0 0.958 0.985 0.995
Peppas-Korsmeyer方程 0.588 1 0.316 0 0.362 9 0.925 0.958 0.925
Higuchi方程 0.226 3 0.208 3 0.217 3 0.432 0.912 0.848

图7

S2中TIBA的累积释放率-时间拟合结果"

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