聚多巴胺涂层聚丙烯疝气补片的制备及其体外炎性反应

doi:10.13475/j.fzxb.20200202205

纺织学报 ›› 2020, Vol. 41 ›› Issue (09): 162-166.doi: 10.13475/j.fzxb.20200202205

• 专栏:生物医用纺织材料及其制品 • 上一篇下一篇

聚多巴胺涂层聚丙烯疝气补片的制备及其体外炎性反应

乔燕莎¹^,², 王茜¹^,², 李彦¹^,²^,³, 桑佳雯¹, 王璐¹^,²^,³()

1.东华大学纺织学院, 上海 201620
2.东华大学纺织面料技术教育部重点实验室, 上海 201620
3.东华大学纺织行业生物医用纺织材料与技术重点实验室, 上海 201620

收稿日期:2020-02-12 修回日期:2020-05-29 出版日期:2020-09-15 发布日期:2020-09-25
通讯作者: 王璐
作者简介:乔燕莎(1994—),女,博士生。主要研究方向为生物医用疝修补片。
基金资助:
国家重点研发计划专项资助项目(2016YFB0303300);教育部创新引智基地111计划项目(B07024);中央高校基本科研业务费专项资金;东华大学研究生创新基金项目(CUSF-DH-D-2020018);上海市大学生创新项目(101-03-0178239)

Preparation and in vitro inflammation evaluation of polydopamine coated polypropylene hernia mesh

QIAO Yansha¹^,², WANG Qian¹^,², LI Yan¹^,²^,³, SANG Jiawen¹, WANG Lu¹^,²^,³()

1. College of Textiles, Donghua University, Shanghai 201620, China
2. Key Laboratory of Textile Science & Technology of Ministry of Education, Donghua University, Shanghai 201620, China
3. Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China

Received:2020-02-12 Revised:2020-05-29 Online:2020-09-15 Published:2020-09-25
Contact: WANG Lu

摘要/Abstract

摘要：

为降低聚丙烯(PP)补片的炎性反应,利用多巴胺氧化自聚合,在PP单丝表面制备了聚多巴胺(PDA)抑炎涂层,并对PDA-PP补片的微观形貌、表面成分、接触角、涂层稳定性、力学性能、体外抗氧化和体外抗炎性能进行了表征与分析。结果表明:PDA可在PP单丝表面形成分布均匀的微纳米颗粒涂层,进而使得PP表面的理化性能呈现出相应的变化;PDA不影响PP补片的力学性能,且抗氧化能力和形貌稳定性均可满足炎性反应初期调控的需求;通过炎性应答标志TNF-α和IL-6的24 h释放量可知,黏附于PDA-PP的巨噬细胞较PP补片分别降低了93%和100%的释放,初步证实PDA涂层可有效降低PP补片的体外炎性反应,进而有望应用于临床评估研究。

关键词: 医用纺织品, 聚丙烯补片, 炎性反应, 聚多巴胺, 抗氧化剂, 抗炎

Abstract:

To reduce the possibility of inflammation with the use of polypropylene (PP) hernia mesh, dopamine was oxidized and self-polymerized for the preparation of an anti-inflammatory polydopamine (PDA) coating on the surface of PP monofilament. The micro-morphology, surface composition, water contact angle, coating stability, mechanical property, in vitro anti-oxidant and anti-inflammatory properties of PDA-PP were characterized and analyzed. The results show that PDA formed a coating of micro-nano particles with uniform distribution on PP fiber surfaces, and the coating changed physicochemical properties of PP mesh. Meanwhile, the original mechanical property of PP mesh is not affected by the PDA coating, and the antioxidant ability and good stability of the PDA coated PP mesh meet the needs of the initial inflammatory regulation. The protein expression level of the inflammation marker TNF-α and IL-6 at 24 h indicate a 93% and 100% lower expression levels for macrophages adhered PDA-PP mesh than that of the PP mesh, respectively. The PDA-PP mesh is expected for clinical evaluation studies next.

Key words: medical textiles, polypropylene mesh, inflammation, polydopamine, anti-oxidant, anti-inflammation

中图分类号:

TS181.8

乔燕莎, 王茜, 李彦, 桑佳雯, 王璐. 聚多巴胺涂层聚丙烯疝气补片的制备及其体外炎性反应[J]. 纺织学报, 2020, 41(09): 162-166.

QIAO Yansha, WANG Qian, LI Yan, SANG Jiawen, WANG Lu. Preparation and in vitro inflammation evaluation of polydopamine coated polypropylene hernia mesh[J]. Journal of Textile Research, 2020, 41(09): 162-166.

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样品	断裂强度/(N·cm^-1)		伸长率/%
样品	横向	纵向	横向	纵向
PP	62.2±1.3	40.8±0.6	28.2±0.5	41.5±1.7
PDA-PP	63.6±2.1	41.6±0.8	27.2±1.1	42.0±0.6

样品	CCK-8吸光度值
PP	0.61±0.05
PDA-PP	0.55±0.04
空白对照样本	0.57±0.06

样品	质量浓度/(pg·mL^-1)
样品	TNF-α	IL-6
PP	189.13±2.13	4.8±0.6
PDA-PP	12.781±4.15	0

聚多巴胺涂层聚丙烯疝气补片的制备及其体外炎性反应

Preparation and in vitro inflammation evaluation of polydopamine coated polypropylene hernia mesh

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