聚氨酯泡沫固定化生物体系对活性蓝4的吸附脱色

doi:10.13475/j.fzxb.20210600808

摘要/Abstract

摘要：

为构建长效作用的生物脱色体系,采用聚氨酯泡沫(PUF)材料对生物吸附剂黄曲霉菌Aspergillus flavus A5p1进行固定化,以蒽醌染料活性蓝4(RB4)为模型底物,开展了生物吸附剂固定化前后的吸附动力学、不同RB4质量浓度和NaCl质量浓度对脱色的影响、重复使用脱色等实验的对比研究。结果表明:菌株经过固定化之后显著加快了对染料RB4的脱色,20 min时对 200 mg/L染料 RB4的吸附率达到77.8％,而未固定化的游离细胞90 min的吸附率为62.8％,脱色过程可采用颗粒内扩散动力学模型来描述;PUF–固定化细胞体系对2 000 mg/L染料RB4的吸附量约是游离细胞体系的2.5倍,并且能耐受NaCl质量浓度为50 g/L 的高盐条件进行脱色;PUF–固定化细胞体系重复使用 7次仍能保持89％的脱色率,而游离细胞体系则下降至74.3％,该PUF–固定化细胞体系展示出对提高RB4生物脱色的有效性及可靠性。

关键词: 生物脱色, 黄曲霉菌, 蒽醌染料, 活性染料, 聚氨酯, 吸附

Abstract:

In order to construct a long-time effect of decolorization system, the biological adsorbent of Aspergillus flavus A5p1 was immobilized on the polyurethane foam (PUF) to decolorize the anthraquinone dye of Reactive Blue 4 (RB4). The adsorption kinetics, different concentrations of RB4, different concentrations of NaCl and repeated batches decolorization by Aspergillus flavus A5p1 before and after immobilization were investigated and compared. Results showed that the decolorization of RB4 was significantly enhanced after Aspergillus flavus A5p1 immobilization. Approximately 77.8％ adsorption of the 200 mg/L RB4 was achieved by the PUF–immobilized biological system within 20 min, while 62.8％ was achieved by the free cell system within 90 min. The biological decolorization process of RB4 could be described by the kinetics model of intraparticle diffusion. It was found that the PUF–immobilized biosystem adsorbed around 2.5 times more RB4 than that of the free cell system when RB4 concentration was 2 000 mg/L. Moreover, the PUF–immobilized biosystem exhibited good tolerance to the salinity of 50 g/L NaCl. In the repeated batches experiment, the decolorization efficiency of PUF–immobilized biological system could be maintained at 89％ after 7 batches, whereas the free cell system was decreased to 74.3％. These results show the effectiveness and reliability of the PUF–immobilized biosystem.

Key words: biological decolorization, Aspergillus flavus, anthraquinone dye, reactive dye, polyurethane, adsorption

中图分类号:

TQ610.9

杨文博, 张傲洁, 刘幽燕, 李青云. 聚氨酯泡沫固定化生物体系对活性蓝4的吸附脱色[J]. 纺织学报, 2022, 43(08): 132-139.

YANG Wenbo, ZHANG Aojie, LIU Youyan, LI Qingyun. Adsorption and decolorization of Reactive Blue 4 by polyurethane foam-immobilized biosystem[J]. Journal of Textile Research, 2022, 43(08): 132-139.

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Langmuir			Freundlich
K_L/(L·mg^–1)	Q_m/(mg·g^–1)	R²	K_F/(L·g^–1)	n	R²
0.010	3.96	0.973	0.425	3.03	0.912

细胞种类	第1阶段			第2阶段			第3阶段
细胞种类	K_i1/ (mg·(g·min^1/2)^–1)	C_i1	R²	K_i2/ (mg·(g·min^1/2)^–1)	C_i2	R²	K_i3/ (mg·(g·min^1/2)^–1)	C_i3	R²
PUF–固定化细胞	60.7	–132.1	0.994	0.901	61.5	0.867	0.041	68.1	0.336
游离细胞	15.3	–30.7	0.995	5.094	6.66	0.975	0.070	48.6	0.724