聚乳酸-己内酯/纤维蛋白原纳米纤维基补片的制备与表征

doi:10.13475/j.fzxb.20200301106

纺织学报 ›› 2021, Vol. 42 ›› Issue (01): 40-45.doi: 10.13475/j.fzxb.20200301106

聚乳酸-己内酯/纤维蛋白原纳米纤维基补片的制备与表征

杨刚¹^,²^,³, 李海迪¹^,², 乔燕莎¹^,², 李彦¹^,²(), 王璐¹^,², 何红兵¹^,³

1.东华大学纺织学院, 上海 201620
2.东华大学纺织面料技术教育部重点实验室, 上海 201620
3.上海松力生物技术有限公司, 上海 201100

收稿日期:2020-03-05 修回日期:2020-07-30 出版日期:2021-01-15 发布日期:2021-01-21
通讯作者: 李彦
作者简介:杨刚(1995—),男,硕士生。主要研究方向为生物医用疝修补片。
基金资助:
国家重点研发计划项目(2016YFB0303300);教育部创新引智基地111计划项目(B07024);东华大学研究生创新基金项目(CUSF-DH-D-2020018)

Preparation and characterization of polylactic acid-caprolactone/fibrinogen nanofiber based hernia mesh

YANG Gang¹^,²^,³, LI Haidi¹^,², QIAO Yansha¹^,², LI Yan¹^,²(), WANG Lu¹^,², HE Hongbing¹^,³

1. College of Textiles, Donghua University, Shanghai 201620, China
2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China
3. Shanghai PINE & POWER Biotech Co., Ltd., Shanghai 201100, China

Received:2020-03-05 Revised:2020-07-30 Online:2021-01-15 Published:2021-01-21
Contact: LI Yan

摘要/Abstract

摘要：

为制备新型疝修补片,将聚乳酸-己内酯(P(LLA-CL))与纤维蛋白原以不同比例共混并纺丝,依托静电纺丝技术制备了一系列P(LLA-CL)/纤维蛋白原复合纳米纤维膜,结合扫描电子显微镜、红外光谱仪、X射线光电子能谱分析仪及细胞实验等对纤维膜片的微观形貌、表面成分、亲水性、力学性能和细胞相容性进行了表征。结果表明:P(LLA-CL)与纤维蛋白原共混后,所制得的纤维膜片均以纳米尺度纤维构成,孔隙率达到60%以上,且膜中存在纤维蛋白原成分;纤维蛋白原的加入使疏水性P(LLA-CL)的亲水性得到大幅提升,并改变了P(LLA-CL)材料原有的力学性能;最终获得的静电纺P(LLA-CL)/纤维蛋白原纳米纤维膜具有良好的细胞相容性,且因其含生长因子,促进了细胞增殖,具有潜在的诱导组织再生能力,有望应用于疝修补领域中。

关键词: 疝修补片, 聚乳酸-己内酯, 纤维蛋白原, 静电纺丝, 医用纺织品

Abstract:

This paper reports on a research on preparation and characterization of a new tissue mesh engineered for hernia repair, where poly(L-lactide-co-caprolactone)/P(LLA-CL) and fibrinogen were blended in different proportions for electro-spinning. A series of P(LLA-CL)/fibrinogen composite nanofiber membranes were prepared, and their micromorphology, surface composition, wettability, mechanical properties and cellular compatibility were characterized and analyzed by scanning electron microscope, Fourier-transformed infrared spectroscopy, X-ray photoelectron spectroscopy, and cell experiments. The results showed that, after combining P(LLA-CL) with fibrinogen, the obtained fibrous membranes were all composed of nanoscale fibers and the porosity reached more than 60%. The addition of fibrinogen greatly improved the hydrophilicity of P(LLA-CL) and changed its original mechanical property. The results show that the P(LLA-CL)/fibrinogen nanofibrous membrane has a good cellular compatibility, and the high level porosity promotes the cell proliferation. In general, the nanofiber based hernia mesh demonstrates a great potential for tissue regeneration, providing a new hope for hernia repair.

Key words: hernia mesh, poly(L-lactide-co-caprolactone), fibrinogen, electrospinning, medical textiles

中图分类号:

TS181.8

杨刚, 李海迪, 乔燕莎, 李彦, 王璐, 何红兵. 聚乳酸-己内酯/纤维蛋白原纳米纤维基补片的制备与表征[J]. 纺织学报, 2021, 42(01): 40-45.

YANG Gang, LI Haidi, QIAO Yansha, LI Yan, WANG Lu, HE Hongbing. Preparation and characterization of polylactic acid-caprolactone/fibrinogen nanofiber based hernia mesh[J]. Journal of Textile Research, 2021, 42(01): 40-45.

图/表 14

表1

图1

图2

表2

图3

表3

图4

表4

图5

表5

图6

表6

图7

表7

参考文献 15

[1]	许洪斌. 全面解读疝气[N]. 卫生与生活报, 2008 -02-01(1).
	XU Hongbin. Comprehensive interpretation of hernia [N]. Journal of Health and Life, 2008 -02-01(1).
[2]	段先召, 王婉东, 陈洪流. 腹股沟疝修补术后慢性疼痛的最新研究进展[J]. 世界最新医学信息, 2018,72:78-82.
	DUAN Xianzhao, WANG Wandong, CHEN Hongliu. Recent advances in chronic pain after inguinal hernia repair[J]. World Latest Medicine Information, 2018,72:78-82.
[3]	NICHOLAS P, ELIE R, JULIE S G. Cancer of unknown primary: incidence rates, risk factors and survival among adolescents and young adults[J]. International Journal of Cancer, 2019,146(6):1490-1498. pmid: 31144291
[4]	ASLANI N, BROWN C J. Does mesh offer an advantage over tissue in the open repair of umbilical hernias systematic review and meta-analysis[J]. Hernia, 2010,14(5):455-462. doi: 10.1007/s10029-010-0705-9 pmid: 20635190
[5]	NEIL J Smart. Biological meshes: a review of their use in abdominal wall hernia repairs[J]. Surgeon, 2012 ( 3):159-171.
[6]	郭盛旗. 人工材料聚丙烯补片在修补腹壁切口疝中的应用[J]. 中国组织工程研究与临床康复, 2010(47):8881-8884.
	GUO Shengqi. Application of artificial material polypropylene patch in repairing abdominal wall incision hernia[J]. Journal of Tissue Engineering Research and Clinical Rehabilitation, 2010 (47):8881-8884.
[7]	陈双, 杨斌. 疝修补的新型材料[J]. 中国微创外科杂志, 2007(12):1123-1124.
	CHEN Shuang, YANG Bin. New materials for hernia repair[J]. Chinese Journal of Minimally Invasive Surgery, 2007 (12):1123-1124.
[8]	BELLOWS C F, JIAN W, M C HALE M K, et al. Blood vessel matrix: a new alternative for abdominal wall reconstruction[J]. Hernia, 2008,12(4):351-358. pmid: 18235999
[9]	陈富强, 申英末. 生物补片在疝和腹壁外科的应用及研究进展[J]. 中华疝和腹壁外科杂志, 2016,10(5):364-368.
	CHEN Fuqiang, SHEN Yingmo. Application and research progress of biologic patch in hernia and abdominal wall surgery[J]. Chinese Journal of Hernia and Abdominal Wall Surgery, 2016,10(5):364-368.
[10]	AGARWAL S, WENDORFF J H, GREINER A. Use of electrospinning technique for biomedical applica-tions[J]. Polymer, 2008,49(26):5603-5621.
[11]	QIN C X, CHENG S, WANG J J, et al. Preparation of nano-fluorescent polyimide fibers by electrospin-ning[J]. Journal of Fiber Bioengineering & Informatics, 2009,2(4):226-230.
[12]	MO X M, XU C Y, KOTAKI M, et al. Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation[J]. Biomaterials, 2004,25(10):1883-1890. doi: 10.1016/j.biomaterials.2003.08.042
[13]	郭明珠. 血浆纤维蛋白原、D-二聚体在AECOPD中的临床应用分析[D]. 长春:吉林大学中日联谊医院, 2016: 37-39.
	GUO Mingzhu. Analysis of clinical application of plasma fibrinogen and D-dimer in AECOPD [D]. Changchun: China-Japan Friendship Hospital of Jilin University, 2016: 37-39.
[14]	王永先, 包斌, 宋瑞瑞. 纤维蛋白原的空间结构特征[J]. 生理科学进展, 2012,43(5):397-401.
	WANG Yongxian, BAO Bin, SONG Ruirui. Spatial structural characteristics of fibrinogen[J]. Advances in Physiological Sciences, 2012,43(5):397-401.
[15]	LEE J, TAE G, KIM Y H, et al. The effect of gelatin incorporation into electrospun poly(L-lactide-co-epsilon-caprolactone) fibers on mechanical properties and cytocompatibility[J]. Biomaterials, 2008,29(12):1872-1879. pmid: 18234330

相关文章 15

[1]	陈云博, 朱翔宇, 李祥, 余弘, 李卫东, 徐红, 隋晓锋. 相变调温纺织品制备方法的研究进展[J]. 纺织学报, 2021, 42(01): 167-174.
[2]	王赫, 王洪杰, 阮芳涛, 凤权. 静电纺聚丙烯腈/线性酚醛树脂碳纳米纤维电极的制备及其性能[J]. 纺织学报, 2021, 42(01): 22-29.
[3]	杨宇晨, 覃小红, 俞建勇. 静电纺纳米纤维功能性纱线的研究进展[J]. 纺织学报, 2021, 42(01): 1-9.
[4]	汪希铭, 程凤, 高晶, 王璐. 交联改性对敷料用壳聚糖/ 聚氧化乙烯纳米纤维膜性能的影响[J]. 纺织学报, 2020, 41(12): 31-36.
[5]	张倩, 毛吉富, 吕璐瑶, 徐仲棉, 王璐. 腱骨修复用缝线在锚钉孔眼处的耐磨性能及其影响因素[J]. 纺织学报, 2020, 41(12): 66-72.
[6]	张亦可, 贾凡, 桂澄, 晋蕊, 李戎. 聚偏氟乙烯/ FeCl₃ 复合纤维膜柔性传感器的制备及其性能[J]. 纺织学报, 2020, 41(12): 13-20.
[7]	刘明洁, 林婧, 关国平, BROCHU G, GUIDOIN R, 王璐. 典型纺织基人工韧带及其移出物结构与力学性能[J]. 纺织学报, 2020, 41(11): 66-72.
[8]	王利媛, 康卫民, 庄旭品, 鞠敬鸽, 程博闻. 磺化聚醚砜纳米纤维复合质子交换膜的制备及其性能[J]. 纺织学报, 2020, 41(11): 19-26.
[9]	李好义, 许浩, 陈明军, 杨涛, 陈晓青, 阎华, 杨卫民. 纳米纤维吸声降噪研究进展[J]. 纺织学报, 2020, 41(11): 168-173.
[10]	王子希, 胡毅. 基于ZnCo₂ O₄ 的多孔碳纳米纤维制备及其储能性能[J]. 纺织学报, 2020, 41(11): 10-18.
[11]	段方燕, 王闻宇, 金欣, 牛家嵘, 林童, 朱正涛. 淀粉纤维的成形及其载药控释研究进展[J]. 纺织学报, 2020, 41(10): 170-177.
[12]	乔燕莎, 王茜, 李彦, 桑佳雯, 王璐. 聚多巴胺涂层聚丙烯疝气补片的制备及其体外炎性反应[J]. 纺织学报, 2020, 41(09): 162-166.
[13]	严佳, 李刚. 医用纺织品的研究进展[J]. 纺织学报, 2020, 41(09): 191-200.
[14]	潘璐, 程亭亭, 徐岚. 聚己内酯/聚乙二醇大孔径纳米纤维膜的制备及其在组织工程支架中的应用[J]. 纺织学报, 2020, 41(09): 167-173.
[15]	杨凯, 张啸梅, 焦明立, 贾万顺, 刁泉, 李咏, 张彩云, 曹健. 高邻位酚醛基纳米活性碳纤维制备及其吸附性能[J]. 纺织学报, 2020, 41(08): 1-8.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

细胞毒性分级	R_GR/%	细胞毒性
0	≥100	无
I	75~99	无
II	50~74	轻度
III	25~49	中度
IV	1~24	中度
V	<1	明显

P(LLA-CL)与纤维蛋白原体积比	孔隙率/%
1:0	56.56±1.32
2:1	63.25±2.07
1:1	69.31±1.77
1:2	71.22±1.94
0:1	72.87±2.31

P(LLA-CL)与纤维蛋白原体积比	含量 /%
P(LLA-CL)与纤维蛋白原体积比	C	N	O	S
1:0	67.71	0.00	32.74	0.00
2:1	69.04	3.35	29.08	0.17
1:1	67.53	4.21	28.55	0.23
1:2	66.38	8.63	25.69	0.29
0:1	65.46	15.12	18.30	0.58

P(LLA-CL)与纤维蛋白原体积比	接触角 /(°)
1:0	137.4±4.5
2:1	83.7±2.1
1:1	59.3±1.0
1:2	34.2±3.1
0:1	19.3±3.3

P(LLA-CL)与纤维蛋白原体积比	断裂强度/ MPa	弹性模量/ MPa	断裂伸长率/%
1:0	8.10±0.14	5.48±0.35	190.23±15.14
2:1	7.61±0.83	8.36±0.76	60.26±3.01
1:1	6.80±0.35	9.59±1.17	54.40±2.81
1:2	2.26±0.18	10.31±0.52	40.93±2.02
0:1	1.36±0.09	15.05±2.14	21.34±4.23

聚乳酸-己内酯/纤维蛋白原纳米纤维基补片的制备与表征

Preparation and characterization of polylactic acid-caprolactone/fibrinogen nanofiber based hernia mesh

RichHTML

PDF (PC)

摘要/Abstract

引用本文

使用本文

图/表 14

参考文献 15

相关文章 15

Metrics

本文评价

推荐阅读 0

样品组别	相对增殖率/%
样品组别	1 d	3 d	5 d
P(LLA-CL)/纤维蛋白原	81.3	87.8	93.8
阴性对照组	82.5	88.8	98.4
阳性对照组	21.6	9.3	7.9

P(LLA-CL)与纤维蛋白原体积比	细胞黏附率 /%
P(LLA-CL)与纤维蛋白原体积比	4 h	24 h	72 h	120 h	168 h
1:0	15.9	19.1	25.3	31.1	34.3
2:1	9.0	18.8	24.2	62.5	78.7
1:1	10.8	20.6	29.6	50.5	69.0
1:2	9.0	17.0	27.1	46.9	59.6
0:1	9.0	18.1	25.6	41.5	62.8