乌拉草提取液中木犀草素含量的测定及其抗菌性能

doi:10.13475/j.fzxb.20200703707

摘要/Abstract

摘要：

为充分开发乌拉草的应用价值,探究其抗菌性能,采用水醇提取法制备了乌拉草提取液,研究了不同醇水比提取液的基本性能。利用紫外分光光度法和高效液相色谱法测试乌拉草提取液中抗菌物质木犀草素的含量,并对2种测试结果进行比较,采用体外抗菌法测试其抑菌率。结果表明:随着提取溶剂中水的比例的增加,提取液的电导率逐渐增大,pH值逐渐减小且均呈现酸性;紫外分光光度法及高效液相色谱法得出的木犀草素含量变化趋势一致,随着提取溶剂中水的比例的增大,木犀草素的含量均逐渐减少;不同醇水比的4种样品对大肠杆菌及金黄色葡萄球菌的抑菌率均大于70%,表明乌拉草提取液具有良好的抗菌性。

关键词: 乌拉草提取液, 紫外分光光度法, 高效液相色谱法, 木犀草素, 体外抗菌活性, 抗菌性能

Abstract:

In order to develop the application of carex meyeriana, and explore its antibacterial performance, the carex meyeriana extract was extracted by alcohol water mixture, and the basic performance of the extract with different alcohol water ratios were tested. The content of luteolin, an antibacterial substance, was tested by ultraviolet (UV) spectrophotometer and high performance liquid chromatography (HPLC) method, and the results of the two tests are compared. Its antibacterial rate was tested by in vitro antibacterial method. The results show that with the increase of the proportion of water in the extraction solvent, the conductivity of the extraction solution gradually increases and the pH value gradually decreases exhibiting acidity. The change trends of luteolin content detected by using UV spectrophotometer and HPLC method are the same. The content of luteolin decreases with the increase of the proportion of water in the extraction solvent, and the antibacterial rates of Escherichia coli and Staphylococcus aureus in the four samples with different alcohol water ratios were all higher than 70%, which indicates that the extract has good antibacterial properties.

Key words: carex meyeriana extract, ultraviolet spectrophotometry method, high performance liquid chromatography method, luteolin, in vitro antibacterial activity, antibacterial property

中图分类号:

TS101.4

王春红, 杨璐, 胡敏, 王晓云, 王利剑. 乌拉草提取液中木犀草素含量的测定及其抗菌性能[J]. 纺织学报, 2021, 42(04): 114-120.

WANG Chunhong, YANG Lu, HU Min, WANG Xiaoyun, WANG Lijian. Determination of luteolin content in carex meyeriana extract and its antibacterial properties[J]. Journal of Textile Research, 2021, 42(04): 114-120.

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培养基名称	牛肉膏质量/g	蛋白胨质量/g	溶剂
培养基名称	牛肉膏质量/g	蛋白胨质量/g	名称	体积/mL
A	0.6	1.0	提取液a	200
B	0.6	1.0	提取液b	200
C	0.6	1.0	提取液c	200
D	0.6	1.0	提取液d	200
E	0.9	1.5	蒸馏水	300

提取液编号	pH值	电导率/(μS·cm^-1)
a	6.39	192
b	6.26	322
c	5.94	471
d	5.69	546

提取液编号	吸光度	木犀草素质量浓度/(μg·mL^-1)
a	1.375	29.22
b	0.123	1.36
c	0.121	1.31
d	0.110	1.07

提取液编号	峰面积/au·min	木犀草素的质量浓度/(μg·mL^-1)
a	3 803 864	37.97
b	182 512	1.48
c	172 481	1.38
d	145 449	1.11

平板序号	细菌计数平均值/(10⁹ CFU·mL^-1)	抑菌率/%
实验组A	4.24	90.6
实验组B	8.73	80.5
实验组C	9.40	79.2
实验组D	11.10	75.5
对照组E	45.30	—