Journal of Textile Research ›› 2025, Vol. 46 ›› Issue (04): 29-37.doi: 10.13475/j.fzxb.20240104901

• Fiber Materials • Previous Articles     Next Articles

Preparation and bovine serum albumin separation of ethylene vinyl alcohol copolymer nanofibrous anion-exchange aerogel

CAO Zhanrui1,2, JI Cancan1,2, HE Shanshan1,2, ZHOU Feng1,3, XIANG Yang3, GAO Fei3, LIU Ke1,2(), WANG Dong1,2   

  1. 1. Key Laboratory of Textile Fibers and Products, Ministry of Education, Wuhan Textile University, Wuhan, Hubei 430200, China
    2. Key Laboratory of Nonwoven Filtration and Separation Materials for Textile Industry, Wuhan Textile University, Wuhan, Hubei 430200, China
    3. Wuhan Branch, Anheuserbusch Enterprise Management (Shanghai) Co., Ltd., Wuhan, Hubei 430051, China
  • Received:2024-01-29 Revised:2024-11-18 Online:2025-04-15 Published:2025-06-11
  • Contact: LIU Ke E-mail:kliu@wtu.edu.cn

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Abstract:

Objective Traditional microsphere chromatography materials have disadvantages in pontificating bovine serum albumin and other biological products with slow separation speed and low separation efficiency. To solve this issue, ethylene vinyl alcohol copolymer (EVOH) nanofibers and chitosan quaternary ammonium salt have been adopted to prepare composite aerogel materials by freeze-drying method. This study provides a simple and efficient method for the construction of biomacromolecule separation and purification materials.

Method EVOH nanofibers were used as support framework material, glutaraldehyde was used as crosslinking agent, and chitosan quaternary ammonium salt was used as modifier and binder to prepare aerogel materials. The surface morphology and surface chemical composition of the composite material were characterized and analyzed using SEM, XPS, FT-IR, and other characterization techniques. The mechanical properties of aerogel materials were characterized by universal tensile testing machine. The hydrophilicity of aerogel materials in dry and wet environment was characterized by contact angle tester. The adsorption capacity test was used to study the BSA adsorption performance of aerogels.

Result Aerogel materials possesses the laminated or spheric structure derived from EVOH nanofibers and chitosan quaternary ammonium salt solution blends in via freeze-drying process. For EVOH/CS-25%, spheric structure has been obtained different to that of other samples with more or less the content of chitosan quaternary ammonium salt.With the decrease of chitosan quaternary ammonium salt, the aerogel materials present decreased water contact angle and shorter time spent to total infusion of water. In addition, as the content of chitosan quaternary ammonium salts decreases, the water absorption of the materials also gradually decreases implying the combined function of chitosan derivative and nanofibrous scaffold. Through the infrared spectra and X-ray photoelectron spectra, it can be seen that aerogel materials have new absorption peaks at 1 645 cm-1, and a wide peak at 3 325 cm-1 different from EVOH nanofibers, which belongs toa vibration caused by the quaternary ammonium group of chitosan quaternary ammonium salts. These results suggest that chitosan quaternary ammonium salts are successfully crosslinked to the EVOH nanofiber surface. It is worth noting that when the content of chitosan quaternary ammonium salt is 17%, the adsorption performance is lower than that of the concentration of 25%, it can be seen that increasing the content of chitosan quaternary ammonium salt is conducive to improving the adsorption performance. Compared with other aerogels with chitosan quaternary ammonium salt content, EVOH/CS-25% has a through-pore structure and good structural stability, so that the saturated adsorption capacity is larger. These results indicate that the adsorption performance of aerogels is jointly affected by the content of chitosan quaternary ammonium salts and the porous structure of nanofiber scaffold.The unique structure of EVOH/CS-25% provides more continuous three-dimensional pores, which is beneficial to the improved mechanical performance to withstand pressure and decompression in air and water indicating a good compression resistance during ion exchange chromatography.

Conclusion A novel aerogel adsorbent was prepared by combining EVOH nanofibers with chitosan quaternary ammonium salts. The nanofiber aerogel materials present a unique microporous network structure and higher specific surface area than the membranes with the same chemical components. The results showed that the composite adsorbent effectively adsorbed the BSA, with a static saturation adsorption capacity of up to 1 121.6 mg/g and a shorter time for saturation adsorption, which can be assigned to the three-dimensional structure of aerogel material with higher specific surface area and interconnected porous channels. Therefore, this work provides a new and efficient approach for the development of anion exchange materials with fast adsorption rate and large adsorption capacity.

Key words: ethylene-vinyl alcohol nanofiber, chitosan quaternary ammonium salt, aerogel, bovine serum albumin

CLC Number: 

  • TH145

Fig.1

Schematic diagram of preparation of EVOH nanofibers"

Fig.2

Chemical reaction formula of EVOH nanofibers and chitosan quaternary ammonium salt crosslinked with glutaraldehyde"

Fig.3

Scanning electron microscopy images of aerogels and EVOH nanofibers. (a) EVOH/CS-50%; (b) EVOH/CS-33%; (c) EVOH/CS-25%; (d) EVOH/CS-17%; (e) EVOH/CS-9%; (f) EVOH nanofibers"

Fig.4

Water contact angle of different samples"

Fig.5

Infrared spectra and X-ray photoelectron spectra of EVOH nanofibers and aerogels with four different contents of chitosan quaternary ammonium salt. (a)Infrared spectra of samples with different chitosan quaternary content; (b) X-ray photoelectron spectra of samples; (c) C1s, O1s and N1s spectra"

Fig.6

Adsorption performance diagram of five groups of aerogel materials for BSA"

Fig.7

Optical photos of EVOH/CS-25% before(a), under(b) and after(c) compression"

Fig.8

Compressive stress-strain curves for EVOH/CS-25% in air (a), water (b) and compressive cycle curves in water (c)"

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