Journal of Textile Research ›› 2024, Vol. 45 ›› Issue (12): 25-32.doi: 10.13475/j.fzxb.20230904201

• Fiber Materials • Previous Articles     Next Articles

Regulation of cell migration and vascularization using electrospun nanofiber yarns

WANG Yawen1,2, LIU Na2, WANG Yuanfei3, WU Tong1,2()   

  1. 1. College of Textile & Clothing, Qingdao University, Qingdao, Shandong 266071, China
    2. Medical College, Qingdao University, Qingdao, Shandong 266071, China
    3. Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, Shandong 266001, China
  • Received:2023-09-17 Revised:2024-06-14 Online:2024-12-15 Published:2024-12-31
  • Contact: WU Tong E-mail:twu@qdu.edu.cn

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Abstract:

Objective Tissue engineering offers a promising therapeutic approach for chronic and acute skin injuries, primarily repairing and regenerating damaged tissues through artificial scaffolds. The migration of human skin fibroblasts (HSFs) and human umbilical vein endothelial cells (HUVECs) plays a crucial role in tissue repair and regeneration. Meanwhile, adipose stem cells (ADSCs) secrete various pro-angiogenic and anti-apoptotic factors essential for tissue repair and regeneration. Therefore, to investigate the effect of the conditioned medium of ADSCs on cell behaviors, three nanofiber yarn-based mesh scaffolds of different sizes were prepared and co-cultured with ADSCs, and the conditioned mediums obtained were co-cultured with HSFs and HUVECs to explore their regulatory effects on the migration and vascularization of these wound repair related cells.

Method The nanofiber yarn-based meshes with different sizes were prepared by light-welding and electrospinning, and the microstructure of different scaffolds was characterized by scanning electron microscopy (SEM) and digital photography, the welding temperatures of nanofiber yarn-based mesh scaffolds were measured by thermographic camera. ADSCs were cultured on scaffolds of different sizes to obtain different conditioned media, and HSFs and HUVECs were cultured with these different conditioned mediums. Cell viability was detected by CCK-8 kit, and cell morphology was observed by fluorescence microscopy.

Results SEM images and digital photographs showed the different sizes of nanofiber yarn-based mesh scaffolds and the uniform size of nanofiber yarns (257.69 ± 36.87) μm which was achieved at the welding temperature (39.83 ± 3.07) ℃. The viability and migration experiments of HSFs showed that the different conditioned mediums of nanofiber yarn-based mesh scaffolds had little effect on cell viability, but their biocompatibilities were improved over that of the control group. The healing rate of HSFs after scratches of small nanofiber yarn-based mesh scaffolds (SNS) and medium nanofiber yarn-based mesh scaffolds (MNS) was better than that of large nanofiber yarn-based mesh scaffolds (LMN) and control group. There was no significant difference in the effect of 3 different conditioned media on the viability of HUVECs among all groups, SNS group had better effect on the migration of HUVECs and SNS group and MNS group promoted the angiogenesis of HUVECs.

Conclusion The conditioned medium obtained after co-culturing ADSCs with nanofiber yarn meshes could effectively promote in vitro migration and angiogenesis of HSFs and HUVECS. Among them, the SNS scaffold was more effective in regulating cell behavior. The modulation of wound healing-related cell behavior utilizing nanofibrous scaffolds cultured with stem cell-collecting conditioned media is expected to be used in wound healing-related applications, providing new ideas for tissue regeneration and repair.

Key words: scaffold material, electrospinning, wound repair, human umbilical vein endothelial cell, human skin fibroblast, adipose stem cell, cell migration, vascularization

CLC Number: 

  • TQ342.87

Fig.1

Flow chart and scheme diagram of experiment"

Fig.2

Photographs (a), SEM images (b) and thermal imaging photos (c) of 3 types of nanofiber yarn-based meshes"

Tab.1

Viability at 3 d and migration rate at 24 h after scratch of HSFs and cultured with different conditions mediums"

试样名称 HSFs 3 d时吸
光度/nm		 HSFs 24 h时
迁移率/%
对照组 0.654 8 ± 0.041 9 10.19 ± 0.25
LNS 0.834 2 ± 0.066 9 47.07 ± 2.83
MNS 0.879 6 ± 0.044 2 49.84 ± 1.89
SNS 0.868 0 ± 0.027 7 57.21 ± 5.20

Fig.3

Light microscope images at 0 h (a) and fluorescence images at 24 h (b) of HSFs cultured in different conditional medium after scratch test"

Fig.4

Light microscope images at 0 h (a) and fluorescence images at 24 h (b) of HUVECs cultured in different conditional medium after scratch test"

Tab.2

Viability at 3 d and migration rate at 24 h after the scratch of HUVECs and cultured with different conditions mediums"

试样名称 HUVECs 3 d时
吸光度/nm		 HUVECs 24 h时
迁移率/%
对照组 0.850 0 ± 0.079 7 26.05 ± 4.68
LNS 0.823 4 ± 0.074 5 48.40 ± 4.56
MNS 0.844 1 ± 0.015 8 47.67 ± 4.27
SNS 0.864 9 ± 0.034 8 65.55 ± 3.00

Fig.5

Light microscopy images (a) and fluorescence images (b) in vitro tubule-formation assay of HUVECs cultured in different conditional mediums for 6 h"

Tab.3

Total branching length and number of nodes of in vitro tubule-formation assay of HUVECs cultured in different conditional mediums for 6 h"

试样名称 HUVECs 6 h后
成管长度/μm		 HUVECs 6 h后
节点数量/个
对照组 22.46 ± 1.15 983.40 ± 57.84
LNS 25.67 ± 2.63 1 081.60 ± 162.81
MNS 29.30 ± 2.00 1 289.80 ± 103.93
SNS 29.61 ± 2.41 1 233.20 ± 74.88
[1] LI J, YU F, CHEN G, et al. Moist-Retaining, Self-recoverable, bioadhesive, and transparent in situ forming hydrogels to accelerate wound healing[J]. ACS Applied Materials & Interfaces, 2020, 12(2): 2023-2038.
[2] PEDRAM RAD Z, MOKHTARI J, ABBASI M. Fabrication and characterization of PCL/zein/gum arabic electrospun nanocomposite scaffold for skin tissue engineering[J]. Materials Science and Engineering: C, 2018, 93: 356-366.
[3] 王曙东, 马倩, 王可, 等. 3D生物打印制备组织工程支架的研究进展[J]. 纺织学报, 2023, 44(3): 210-220.
WANG Shudong, MA Qian, WANG Ke, et al. Research progress in tissue engineering scaffolds by 3D bioprinting[J]. Journal of Textile Research, 2023, 44(3): 210-220.
[4] AN Y, LIN S, TAN X, et al. Exosomes from adipose-derived stem cells and application to skin wound hea-ling[J]. Cell Proliferation, 2021. DOI: 10.1111/jocd.13215.
[5] GIZAW M, FAGLIE A, PIEPER M, et al. The role of electrospun fiber scaffolds in stem cell therapy for skin tissue regeneration[J]. Med One, 2019. DOI: 10.20900/mo.20190002.
[6] HAO Z, QI W, SUN J, et al. Review: research progress of adipose-derived stem cells in the treatment of chronic wounds[J]. Frontiers in Chemistry, 2023. DOI: 10.3389/fchem.2023.1094693.
[7] SCHNEIDER I, CALCAGNI M, BUSCHMANN J. Adipose-derived stem cells applied in skin diseases, wound healing and skin defects: a review[J]. Cytotherapy, 2023, 25(2): 105-119.
[8] BORGESE M, BARONE L, ROSSI F, et al. Effect of nanostructured scaffold on human adipose-derived stem cells: outcome of in vitro experiments[J]. Nanomaterials, 2020. DOI: 10.3390/nano10091822.
[9] LIN L, XU Y, LI Y, et al. Nanofibrous Wharton's jelly scaffold in combination with adipose-derived stem cells for cartilage engineering[J]. Materials & Design, 2020. DOI: 10.1016/j.matdes.2019.108216.
[10] NING X, LIU N, SUN T, et al. Promotion of adipose stem cell transplantation using GelMA hydrogel reinforced by PLCL/ADM short nanofibers[J]. Biomedical Materials, 2023. DOI: 10.1088/1748-605x/acf551.
[11] HUANG W, XIAO Y, SHI X. Construction of electrospun organic/inorganic hybrid nanofibers for drug delivery and tissue engineering applications[J]. Advanced Fiber Materials, 2019, 1(1): 32-45.
[12] DONG Y, ZHENG Y, ZHANG K, et al. Electrospun nanofibrous materials for wound healing[J]. Advanced Fiber Materials, 2020, 2(4): 212-227.
[13] NULTY J, FREEMAN F E, BROWE D C, et al. 3D bioprinting of prevascularised implants for the repair of critically-sized bone defects[J]. Acta Biomaterialia, 2021, 126: 154-169.
[14] BACAKOVA L, ZARUBOVA J, TRAVNICKOVA M, et al. Stem cells: their source, potency and use in regenerative therapies with focus on adipose-derived stem cells - a review[J]. Biotechnology Advances, 2018, 36(4): 1111-1126.
[15] YAO Q, COSME J G L, XU T, et al. Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone forma-tion[J]. Biomaterials, 2017, 115: 115-127.
[16] CHEN S, LI R, LI X, et al. Electrospinning: An enabling nanotechnology platform for drug delivery and regenerative medicine[J]. Advanced Drug Delivery Reviews, 2018, 132: 188-213.
[17] 付征, 穆齐峰, 张青松, 等. 胶体静电纺微纳米纤维的研究进展[J]. 纺织学报, 2023, 44(10): 196-204.
FU Zheng, MU Qifeng, ZHANG Qingsong, et al. Research progress in colloidal electrospun micro/nano fibers[J]. Journal of Textile Research, 2023, 44(10): 196-204.
[18] XIE J, MACEWAN M R, LI X, et al. Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties[J]. ACS Nano, 2009, 3(5): 1151-1159.
[19] XUE J, WU T, DAI Y, et al. Electrospinning and electrospun nanofibers: methods, materials, and applications[J]. Chemical Reviews, 2019, 119(8): 5298-5415.
[20] HEIDARI M, BAHRAMI H, RANJBAR-MOHAMMADI M. Fabrication, optimization and characterization of electrospun poly(caprolactone)/gelatin/graphene nanofibrous mats[J]. Materials Science and Engineering: C, 2017, 78: 218-229.
[21] 贾姣, 郑作保, 吴昊, 等. 静电纺聚合物复合金属有机框架功能纳米纤维膜的研究进展[J]. 纺织学报, 2023, 44(6): 215-224.
JIA Jiao, ZHENG Zuobao, WU Hao, et al. Research progress in electrospinning functional nanofibers with metal-organic framework[J]. Journal of Textile Research, 2023, 44(6): 215-224.
[22] TASKIN M B, AHMAD T, WISTLICH L, et al. Bioactive electrospun fibers: fabrication strategies and a critical review of surface-sensitive characterization and quantification[J]. Chemical Reviews, 2021, 121(18): 11194-11237.
[23] XUE J, PISIGNANO D, XIA Y. Maneuvering the migration and differentiation of stem cells with electrospun nanofibers[J]. Advanced Science, 2020, 7(15): 2000735.
[24] LIANG R, ZHAO J, LI B, et al. Implantable and degradable antioxidant poly(ε-caprolactone)-lignin nanofiber membrane for effective osteoarthritis treat-ment[J]. Biomaterials, 2020. DOI: 10.1016/j.biomaterials.2019.119601.
[25] SAWADKAR P, MOHANAKRISHNAN J, RAJASEKAR P, et al. A synergistic relationship between polycaprolactone and natural polymers enhances the physical properties and biological activity of sca-ffolds[J]. ACS Applied Materials & Interfaces, 2020, 12(12): 13587-13597.
[26] FENG Z, ZHANG X, LIU N, et al. Promotion of neurite outgrowth and extension using injectable welded nanofibers[J]. Chemical Research in Chinese Universities, 2021, 37(3): 522-527.
[27] WU T, XUE J, XIA Y. Engraving the surface of electrospun microfibers with nanoscale grooves promotes the outgrowth of neurites and the migration of schwann cells[J]. Angewandte Chemie International Edition, 2020, 59(36): 15626-15632.
[28] LIU Y, ZHANG X, WANG Y, et al. Promoting neurite outgrowth and neural stem cell migration using aligned nanofibers decorated with protrusions and galectin-1 coating[J]. Chemical Communications, 2023, 59(72): 10753-10756.
[29] KALIRAJAN C, BEHERA H, SELVARAJ V, et al. In vitro probing of oxidized inulin cross-linked collagen-ZrO2 hybrid scaffolds for tissue engineering applica-tions[J]. Carbohydrate Polymers, 2022. DOI: 10.1016/j.carbpol.2022.119458.
[30] CHEN X, ZHANG L, CHAI W, et al. Hypoxic microenvironment reconstruction with synergistic biofunctional ions promotes diabetic wound healing[J]. Advanced Healthcare Materials, 2023. DOI: 10.1002/adhm.202301984.
[31] DENG S, LEI T, CHEN H, et al. Metformin pre-treatment of stem cells from human exfoliated deciduous teeth promotes migration and angiogenesis of human umbilical vein endothelial cells for tissue enginee-ring[J]. Cytotherapy, 2022, 24(11): 1095-1104.
[32] WU T, LI H, XUE J, et al. Photothermal welding, melting, and patterned expansion of nonwoven mats of polymer nanofibers for biomedical and printing applications[J]. Angewandte Chemie International Edition, 2019, 58(46): 16416-16421.
[33] CHOI J K, JANG J H, JANG W H, et al. The effect of epidermal growth factor (EGF) conjugated with low-molecular-weight protamine (LMWP) on wound healing of the skin[J]. Biomaterials, 2012, 33(33): 8579-8590.
[34] LIU Y, ZHU Z, PEI X, et al. ZIF-8-modified multifunctional bone-adhesive hydrogels promoting angiogenesis and osteogenesis for bone regenera-tion[J]. ACS Applied Materials & Interfaces, 2020, 12(33): 36978-36995.
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