采用还原剂-甲酸法溶解制备羊毛角蛋白质溶液

doi:10.13475/j.fzxb.20180400207

纺织学报 ›› 2019, Vol. 40 ›› Issue (03): 1-7.doi: 10.13475/j.fzxb.20180400207

• 纤维材料 • 下一篇

采用还原剂-甲酸法溶解制备羊毛角蛋白质溶液

李博¹, 姚金波²^,³(), 牛家嵘¹, 王乐¹, 冯懋¹, 孙艳丽¹

1.天津工业大学纺织科学与工程学院, 天津 300387
2.武汉纺织大学化学与化工学院, 湖北武汉 430073
3.武汉纺织大学湖北省生物质纤维与生态染整重点实验室, 湖北武汉 430073

收稿日期:2018-04-02 修回日期:2018-11-30 出版日期:2019-03-15 发布日期:2019-03-15
通讯作者: 姚金波
作者简介:李博(1988—),男,博士生。主要研究方向为纤维结构与性能。
基金资助:
国家科技支撑计划项目(2014BAE01B02)

Preparation of wool keratin solution by reducing agent-formic acid process

LI Bo¹, YAO Jinbo²^,³(), NIU Jiarong¹, WANG Le¹, FENG Mao¹, SUN Yanli¹

1. School of Textile Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China
2. College of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, Hubei 430073, China
3. Hubei Key Laboratory of Biomass and Eco-Dyeing & Finishing, Wuhan Textile University, Wuhan, Hubei 430073, China

Received:2018-04-02 Revised:2018-11-30 Online:2019-03-15 Published:2019-03-15
Contact: YAO Jinbo

摘要/Abstract

摘要：

为获得纤维溶解率高且蛋白质分子质量大的羊毛角蛋白质溶液,对溶解纤维的工艺方法进行研究和优化。在采用三羟基有机磷还原剂有效打开羊毛纤维分子内二硫键的基础上,重点研究了还原处理后羊毛纤维在甲酸溶液中的溶解状态。对纤维溶解率、角蛋白质溶液黏度和蛋白质分子质量分布等进行测试,获得还原剂-甲酸法溶解制备角蛋白质溶液的最优工艺,并分析了该角蛋白质溶液的稳定性能。结果表明:在角蛋白质溶液的最优制备工艺条件下,即预处理后的羊毛纤维质量为5 g,甲酸体积为100 mL,溶解温度为50 ℃,溶解时间为5 h时,羊毛纤维的溶解率为65%左右,角蛋白质的分子质量分布主要集中在40~50 ku、26 ku、14.4 ku处,且在常温环境下该羊毛角蛋白质溶液的稳定性较好。

关键词: 羊毛, 角蛋白质溶液, 还原剂, 甲酸, 蛋白质分子质量

Abstract:

In order to obtain the wool keratin solution with high fiber dissolution ratio and large protein molecular weight, the dissolution method of wool fibers was studied and optimized. On the basis of using organic phosphonic compounds (LKS-610) to effectively cleave the disulfide bonds in fibers, the dissolution state of pre-treated wool fibers in formic acid solution was studied. The fibers dissolution ratio, keratin solution viscosity and protein molecular weight distribution were tested as evaluation criteria, the optimal process for preparing keratin solution by using reducing agent-formic acid method was obtained, and the stability performance of keratin solution was also analyzed. The optimal dissolving process of wool fibers is that the mass of the pretreated wool fibers is 5 g, the volume of formic acid is 100 mL, the temperature is 50 ℃ and time is 5 h. Under the conditions, the dissolution ratio is about 65% and the molecular weights of keratin are distributed at 40-50 ku, 26 ku and 14.4 ku. In addition, the keratin solution shows good stability at normal temperature.

Key words: wool, keratin solution, reducing agent, formic acid, protein molecular weight

中图分类号:

TS195.5

李博, 姚金波, 牛家嵘, 王乐, 冯懋, 孙艳丽. 采用还原剂-甲酸法溶解制备羊毛角蛋白质溶液[J]. 纺织学报, 2019, 40(03): 1-7.

LI Bo, YAO Jinbo, NIU Jiarong, WANG Le, FENG Mao, SUN Yanli. Preparation of wool keratin solution by reducing agent-formic acid process[J]. Journal of Textile Research, 2019, 40(03): 1-7.

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氨基酸名称	未处理羊毛		处理30 min羊毛		处理60 min羊毛
氨基酸名称	质量/ (g·(100 g)^-1)	占氨基酸百分比/%	质量/ (g·(100 g)^-1)	占氨基酸百分比/%	质量/ (g·(100 g)^-1)	占氨基酸百分比/%
天门冬氨酸	5.164 2	6.26	5.427 2	7.46	5.283 8	7.59
苏氨酸	4.830 5	5.88	4.174 5	5.74	3.897 3	5.60
丝氨酸	7.030 1	8.52	5.970 2	8.21	5.603 0	8.05
谷氨酸	11.074 4	13.42	11.209 0	15.42	10.819 0	15.54
甘氨酸	4.407 4	5.34	3.896 1	5.36	3.645 3	5.24
丙氨酸	3.342 3	4.05	4.474 2	6.15	4.673 6	6.71
胱氨酸	8.325 0	10.09	1.123 7	1.55	0.732 3	1.05
缬氨酸	3.990 8	4.83	3.701 1	5.09	3.440 8	4.94
蛋氨酸	0.627 3	0.76	0.497 1	0.68	0.415 6	0.60
异亮氨酸	2.664 2	3.23	2.552 4	3.51	2.484 0	3.57
亮氨酸	6.958 6	8.43	7.016 1	9.65	6.907 8	9.92
酪氨酸	4.257 3	5.16	3.748 4	5.16	3.572 4	5.13
苯丙氨酸	3.173 6	3.84	2.604 7	3.58	2.631 0	3.78
赖氨酸	2.865 8	3.47	2.926 5	4.03	2.957 0	4.25
NH₃(色氨酸分解)	1.241 7	1.50	1.179 5	1.62	1.130 5	1.62
组氨酸	0.941 7	1.14	0.846 3	1.16	0.806 4	1.16
精氨酸	7.870 2	9.53	7.990 8	10.99	7.777 1	11.17
脯氨酸	3.775 5	4.57	3.369 1	4.63	2.825 1	4.06
氨基酸总量	82.545 3	100.00	72.706 8	100.00	69.602 1	100.00

溶液中羊毛质量/g	纤维溶解率/%	溶液黏度/(Pa·s)
3	72.3	0.023
4	70.6	0.040
5	66.7	0.054
6	—	—

溶解温度/ ℃	纤维溶解率/%	溶液黏度/(Pa·s)
20	52.9	0.015
30	54.7	0.052
40	56.3	0.045
50	63.5	0.066
60	67.2	0.048
70	72.9	0.030
80	80.6	0.014

纤维溶解时间/h	纤维溶解率/%	溶液黏度/(Pa·s)
3	47.9	0.047
4	52.7	0.050
5	62.5	0.060
6	68.9	0.039

采用还原剂-甲酸法溶解制备羊毛角蛋白质溶液

Preparation of wool keratin solution by reducing agent-formic acid process

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