Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (11): 41-47.doi: 10.13475/j.fzxb.20200301007

• Fiber Materials • Previous Articles     Next Articles

Structure and biocompatibility of silk fibroin/gelatin blended hydrogels

WANG Shudong1,2,3, MA Qian1, WANG Ke1,4, QU Caixin1(), QI Yu2   

  1. 1. School of Textile and Clothing, Yancheng Polytechnic College, Yancheng, Jiangsu 224005, China
    2. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215002, China
    3. Jiangsu Jinmaisui New Energy Technology Co., Ltd., Yancheng, Jiangsu 224005, China
    4. College of Textile and Clothing, Qingdao University, Qingdao, Shandong 262127, China
  • Received:2020-03-04 Revised:2020-08-12 Online:2020-11-15 Published:2020-11-26
  • Contact: QU Caixin E-mail:qcx001@126.com

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Abstract:

To address the difficulty in silk fibroin curing, gelatin and silk fibroin aqueous solution were blended, and the silk fibroin/gelatin blended hydrogels were prepared by three-dimensional printing. The rheological properties, microstructure and biological properties of the blended hydrogels were investigated. The results show that addition of gelatin increases the viscosity and energy storage modulus of blended hydrogels, which is good for the gel forming in the subsequent three-dimensional printing process. Addition of gelatin does not affect the secondary structure of silk fibroin, and the microperiodic silk fibroin/gelatin blended hydrogels were prepared by three-dimensional printing. The breaking strength of the blended scaffold (blending ratio of silk protein to gelatin was 50∶50) is 3.43 MPa, which is 3.9 times higher than that of the pure silk protein. The printed scaffold has a three-dimensional porous structure after freeze-drying. After 7 days' culturing, MC3T3-E1 cells were found to be able to grow, proliferate and differentiate on the blended hydrogel scaffolds.

Key words: silk fibroin, gelatin, composite hydrogel, three-dimensional printing, biocompatibility, scaffold material, biomedical material

CLC Number: 

  • TS102.512

Fig.1

Morphology (a) and rheological property (b) of silk fibroin/gelatin blended hydrogel with different blended ratios"

Fig.2

Elastic shear and viscous moduli of silk fibroin/gelatin blended hydrogel with different blended ratio"

Fig.3

Mechanical property of silk fibroin/gelatin blended hydrogel"

Fig.4

Microstructure of silk fibroin/gelatin blended hydrogel. (a) Circular dichroism spectra; (b) FT-IR spectra;(c) XRD spectra"

Fig.5

Three-dimensional printing silk fibroin/gelatin blended hydrogels scaffolds with different blended ratios"

Fig.6

Three-dimensional printing silk fibroin/gelatin blended hydrogels freeze-dried scaffolds with different blended ratios"

Fig.7

SEM images of freeze-dried silk fibroin/gelatin blended hydrogel scaffolds with different blended ratios(×100)"

Tab.1

Proliferation of MC3T3-E1 cell on silk fibroin and silk fibroin/gelatin scaffolds"

培养时间/d 吸光度
蚕丝蛋白支架 蚕丝蛋白/明胶
复合水凝胶支架
1 0.130±0.027 0.153±0.014
3 0.172±0.031 0.246±0.009
7 0.250±0.019 0.336±0.013

Fig.8

SEM images of MC3T3-E1 cell on silk fibroin scaffolds (a) and silk fibroin/gelatin scaffolds (b) after 7 days culturing(×200)"

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