Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (04): 20-27.doi: 10.13475/j.fzxb.20210307608

• Fiber Materials • Previous Articles     Next Articles

Effect of ultrasonic treatment on composition and structure of rabbit hair keratin

WANG Xiaoqing1,2, SHI Zhiming2(), LI Xiaoyu1   

  1. 1. College of Textile and Light Industry, Inner Mongolia University of Technology, Hohhot, Inner Mongolia 010080, China
    2. School of Materials Science and Engineering, Inner Mongolia University of Technology, Hohhot, Inner Mongolia 010051, China
  • Received:2021-03-18 Revised:2021-10-29 Online:2022-04-15 Published:2022-04-20
  • Contact: SHI Zhiming E-mail:shizm@imut.edu.cn

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Abstract:

In view of the destruction of disulfide bonds by increasing chemical reagents or high temperature treatment, resulting in the hydrolysis of amide bonds to generate small molecular polypeptides, ultrasonic treatment combined with reduction method was used to extract the keratin from the rabbit hair. The effects of ultrasonic treatment on the chemical composition and structure of rabbit hair keratin were investigated by protein electrophoresis meter, infrared spectrometer, fluorescence spectrometer and particle size analyzer. The results show that with the increase of ultrasonic treatment time, the amino acid composition of rabbit hair keratin remained constant, but the amino acid content decreased. The loss rate of cystine increased and the content of free sulfhydryl group firstly increases and then decreases, and the molecular weight increases gradually and mainly distribute in the range of 31-43 ku. The secondary structure changes from α-helix structure to β-sheet structure and random coil structure, and the particle size of the keratin decrease. The fluorescence spectra show that keratin has fixed structure and is not easy to agglomerate.

Key words: ultrasonic treatment, rabbit hair keratin, amino acid composition, molecular weight, α-helix, β-sheet, chemical structure

CLC Number: 

  • TS252.1

Fig.1

Analysis result of amino acid content"

Tab.1

Contents of different kinds of amino acids"

试样
编号		 酸性氨基
酸含量/%		 碱性氨基
酸含量/%		 中性氨基
酸含量/%		 总含
量/%
RT 19.23 13.31 61.41 93.95
UT-RH 18.62 12.95 59.42 90.99
UT-0 20.47 11.70 53.28 85.45
UT-1 21.25 11.69 53.88 86.82
UT-2 21.55 11.89 54.63 88.07
UT-3 21.22 12.14 54.94 88.30
UT-4 21.53 12.09 54.34 87.96

Fig.2

Effect of ultrasonic treatment on Gys and free sulfhydryl groups of keratins"

Fig.3

SDS-PAGE image of extracted rabbit hair keratin"

Fig.4

Infrared spectroscopy (a) and Raman spectroscopy (b) of rabbit hair keratin"

Tab.2

Secondary structure content of rabbit hair and keratin%"

试样
编号		 α-螺
旋		 β-折
叠		 β-折叠/
无规卷曲		 末端羧
基		 无规卷
曲
RH 43.73 9.66 38.98 7.63 —
UT-RH 7.63 7.63 49.86 3.37 —
UT-0 26.99 18.03 45.14 9.83 —
UT-1 33.52 15.23 44.15 7.11 —
UT-2 29.53 17.53 44.20 8.74 —
UT-3 — 10.72 48.36 11.86 29.06
UT-4 — 7.47 53.33 8.25 30.95

Fig.5

X-ray diffraction for rabbit hair and keratin"

Tab.3

Crystallinity index and lattice spacing"

试样
编号		 结晶
度/%		 衍射峰1 衍射峰2
2θ/(°) 晶面间距/nm 2θ/(°) 晶面间距/nm
RH 21.88 9.47 0.932 20.44 0.434
UT-RH 15.94 9.27 0.953 19.28 0.460
UT-0 2.85 9.31 0.949 19.42 0.456
UT-1 1.56 8.69 1.016 19.80 0.447
UT-2 1.08 9.07 0.974 19.62 0.452
UT-3 1.58 8.97 0.985 19.52 0.454
UT-4 1.19 8.49 1.040 19.58 0.453

Fig.6

Endogenous fluorescence spectra of rabbit hair keratin"

Fig.7

Particle size(a) and ζ-potential(b) of rabbit hair keratin"

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