Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (04): 15-19.doi: 10.13475/j.fzxb.20210302305

• Fiber Materials • Previous Articles     Next Articles

Hemostasis properties of silk fibroin materials under different types of hydrolysis

LEI Caihong1,2, YU Linshuang1, ZHU Hailin1,2, ZHENG Tao1, CHEN Jianyong1,2()   

  1. 1. College of Textile Science and Engineering(International Institute of Silk), Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
    2. Zhejiang Provincial Key Laboratory of Fiber Materials and Manufacturing Technology, Hangzhou, Zhejiang 310018, China
  • Received:2021-03-05 Revised:2022-01-19 Online:2022-04-15 Published:2022-04-20
  • Contact: CHEN Jianyong E-mail:cjy@zstu.edu.cn

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Abstract:

Aiming at the improvement of hemostasis properties of silk fibroin materials fabricated by salt hydrolysis and enzyme hydrolysis method, the silk fibroin materials were prepared via a freeze-drying method. The molecular weight, water solubility and hemostatic effect of the resultant material were analyzed by gel filtration chromatography, water solubility test, coagulation factor test, platelet adhesion test, enzyme-linked immunosorbent assay and rat liver injury model. The results showed that compared with the salt hydrolysis method, the enzyme hydrolysis method could endow a lower molecular weight and better water soluble, which is conducive to activating the coagulation factor XII to promote endogenous coagulation. Moreover, the platelet adhesion and platelet factor 4 content increase significantly, the amount of bleeding from the rat liver is reduced with shortened bleeding time, indicating better hemostatic effect than that of Yunnan white medicine.

Key words: silk, silk fibroin, types of hydrolysis, salt hydrolysis, enzyme hydrolysis, hemostasis property

CLC Number: 

  • TQ341.5

Fig.1

APTT and PT values for two different hydrolysis systems of silk fibroin materials"

Fig.2

Absorbance value of silk fibroin materials produced by two different hydrolysis systems"

Fig.3

Hemostatic time and amount of bleeding for silk fibroin materials produced by two different hydrolysis systems in liver trauma model"

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