Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (12): 7-12.doi: 10.13475/j.fzxb.20200406206

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• Fiber Materials • Previous Articles     Next Articles

Negatively enhanced modification of silk fibroin and its load ability to calcitonin gene-related peptide

SONG Guangzhou1, TU Fangfang1, DING Mengyao1, DAI Mengnan1, YIN Yin2, DONG Fenglin3, WANG Jiannan1,4()   

  1. 1. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China
    2. Medical College of Soochow University, Suzhou, Jiangsu 215123, China
    3. The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China
    4. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
  • Received:2020-04-22 Revised:2020-07-23 Online:2020-12-15 Published:2020-12-23
  • Contact: WANG Jiannan E-mail:wangjn@suda.edu.cn

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Abstract:

In order to improve the rapid endothelialization of silk fibroin (SF) materials, and prevent thrombosis and intimal hyperplasia in vascular tissue engineering, calcitonin gene-related peptide (CGRP), which has the function of bidirectional regulation of vascular cell growth, was loaded onto the surface of SF materials through electrostatic interaction. Adipic acid (AA) containing two carboxyl groups was used to react with SF molecules, and AA modified SF film was prepared by fluidization method. The structure of modified SF films and the loading capacity of CGRP on the film surface were studied. The results show that the Zeta potential of SF gradually decreases with the increase of AA mass ratio. When the mass ratio of SF and AA is changed from 100:0 to 100:2.5, the Zeta potential of SF decreases from -2.66 mV to -5.4 mV. The introduction of new carboxyl groups and amide bonds into AA modified SF materials forms β-sheets and Silk II crystal structure. When SF and AA mass ratio increases to 100:2.5, the content of CGRP loaded by electrostatic action get 9 times higher than that of unmodified SF film surface.

Key words: Bombyx mori silk, silk fibroin, adipic acid, calcitonin gene-related peptide, electrostatic interaction

CLC Number: 

  • TS101.4

Fig.1

Reaction mechanism of AA modified SF"

Fig.2

Zeta potential of AA modified SF in aqueous solution"

Fig.3

Hot water loss rate of AA modified SF films. (a)BSA standard curve; (b)Loss rate of SF"

Fig.4

Infrared spectra of 4 000~2 000 cm-1 (a) and 2 000~1 000 cm-1 (b) of AA modified SF films"

Fig.5

X-ray diffraction curves of AA modified SF films"

Fig.6

Schematic diagram of CGRP loading on AA modified SF films"

Fig.7

Loading capacity of CGRP on AA modified SF films"

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