Journal of Textile Research ›› 2021, Vol. 42 ›› Issue (02): 41-46.doi: 10.13475/j.fzxb.20201008606

• Fiber Materials • Previous Articles     Next Articles

Protein adsorption and cell response on bio-interfaces of silk fibroin/octacalcium phosphate composites

YANG Ya1,2, YAN Fengyi2, WANG Hui2, ZHANG Keqin2()   

  1. 1. School of Textile Garment and Design, Changshu Institute of Technology, Changshu, Jiangsu 215500, China
    2. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215000, China
  • Received:2020-10-30 Revised:2020-11-13 Online:2021-02-15 Published:2021-02-23
  • Contact: ZHANG Keqin E-mail:kqzhang@suda.edu.cn

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Abstract:

To further understand the structure-function relationships for biomimetic materials for the purpose of designing a favorable implant microenvironment for osseointegration in vivo, the nano/micro hierarchical structure of silk fibroin (SF)/octacalcium phosphate (OCP) composite coating was constructed by controlling simply the SF concentration of the electrolyte during the electrochemical deposition process. The influence of SF concentration on surface morphology, mechanical property, protein adsorption and cell proliferation were studied. The results show that the pore diameter of the coating surface decreases from (19.96±6.96) μm to (1.56±0.22) μm and the crystal width decreases to the nano-scale with SF concentration increasing in electrolyte. Comparing the pure OCP coating, the elastic modulus and hardness of the SF/OCP composite coating (1.0 mg/mL SF) increase approximately 1.5 times and 4.3 times, respectively. The SF/OCP composite coating selectively enhances fibronectin (Fn) adsorption, and the cell viability on the SF/OCP composite coating (1.0 mg/mL SF) is 1.28 times that on the pure OCP coating after culturing for 7 days.

Key words: silk fibroin, octacalcium phosphate, protein adsorption, cell culture, biomimetic material

CLC Number: 

  • TS101.4

Fig.1

SEM images of pure OCP coating and SF/OCP composite coatings"

Fig.2

Enlarged SEM images of pure OCP coating and SF/OCP composite coatings"

Fig.3

Change of pore size (a) and OCP crystals width (b) in prepared coatings with SF concentration"

Fig.4

Schematic illustration of formation of SF/OCP composite coatings on Ti substrates by ECD technique"

Tab.1

Elastic modulus and hardness values of prepared coatingsGPa"

样品名称 弹性模量 硬度
OCP涂层 8.56 ± 0.62 0.011 ± 0.001
SF/OCP涂层 12.84 ± 1.34 0.047 ± 0.011

Fig.5

BSA adsorption on pure Ti,pure OCP coating and SF/OCP composite coatings"

Fig.6

Fn adsorption on pure Ti,pure OCP coating and SF/OCP composite coatings"

Fig.7

HUMSCs cell viability on pure OCP coating and SF/OCP composite coatings for 1, 3 and 7 d"

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