丝素蛋白负电性增强改性及其对降钙素基因相关肽的加载能力

doi:10.13475/j.fzxb.20200406206

纺织学报 ›› 2020, Vol. 41 ›› Issue (12): 7-12.doi: 10.13475/j.fzxb.20200406206

所属专题：医用纺织品

丝素蛋白负电性增强改性及其对降钙素基因相关肽的加载能力

宋广州¹, 涂芳芳¹, 丁梦瑶¹, 戴梦男¹, 殷音², 董凤林³, 王建南¹^,⁴()

1.苏州大学现代丝绸国家工程实验室, 江苏苏州 215123
2.苏州大学医学部, 江苏苏州 215123
3.苏州大学附属第一医院, 江苏苏州 215006
4.苏州大学纺织与服装工程学院, 江苏苏州 215123

收稿日期:2020-04-22 修回日期:2020-07-23 出版日期:2020-12-15 发布日期:2020-12-23
通讯作者: 王建南
作者简介:宋广州(1995—),男,硕士生。主要研究方向为生物医用材料。
基金资助:
国家自然科学基金项目(51873141);国家自然科学基金项目(51473108);江苏省自然科学基金项目(BK20181192);江苏省高等学校自然科学研究重大项目(18KJA540001)

Negatively enhanced modification of silk fibroin and its load ability to calcitonin gene-related peptide

SONG Guangzhou¹, TU Fangfang¹, DING Mengyao¹, DAI Mengnan¹, YIN Yin², DONG Fenglin³, WANG Jiannan¹^,⁴()

1. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China
2. Medical College of Soochow University, Suzhou, Jiangsu 215123, China
3. The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China
4. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China

Received:2020-04-22 Revised:2020-07-23 Online:2020-12-15 Published:2020-12-23
Contact: WANG Jiannan

摘要/Abstract

摘要：

为提高丝素蛋白(SF)材料的快速内皮化能力,解决血管组织工程面临的血栓和内膜增生难题,将具有双向调控血管细胞生长功能的降钙素基因相关肽(CGRP)通过静电相互作用引入丝素蛋白材料表面。首先选用含有2个羧基的己二酸(AA)与丝素蛋白分子进行反应,采用流延法制备AA改性SF膜,研究了改性SF膜的结构和膜表面对CGRP分子的加载能力。结果表明:随着AA质量占比的增加,SF的Zeta电位逐渐减小,当SF与AA的质量比由100:0变化为100:2.5时,SF的Zeta电位由-2.66 mV 下降至-5.4 mV;AA改性SF材料中引入了新的羧基和酰胺键,形成了以β-折叠结构为主的分子构象和Silk Ⅱ结晶结构;当SF与AA的质量比为100:2.5时,AA改性SF膜表面静电作用加载的CGRP含量比未改性SF膜提高了9倍。

关键词: 家蚕生丝, 丝素蛋白, 己二酸, 降钙素基因相关肽, 静电相互作用

Abstract:

In order to improve the rapid endothelialization of silk fibroin (SF) materials, and prevent thrombosis and intimal hyperplasia in vascular tissue engineering, calcitonin gene-related peptide (CGRP), which has the function of bidirectional regulation of vascular cell growth, was loaded onto the surface of SF materials through electrostatic interaction. Adipic acid (AA) containing two carboxyl groups was used to react with SF molecules, and AA modified SF film was prepared by fluidization method. The structure of modified SF films and the loading capacity of CGRP on the film surface were studied. The results show that the Zeta potential of SF gradually decreases with the increase of AA mass ratio. When the mass ratio of SF and AA is changed from 100:0 to 100:2.5, the Zeta potential of SF decreases from -2.66 mV to -5.4 mV. The introduction of new carboxyl groups and amide bonds into AA modified SF materials forms β-sheets and Silk II crystal structure. When SF and AA mass ratio increases to 100:2.5, the content of CGRP loaded by electrostatic action get 9 times higher than that of unmodified SF film surface.

Key words: Bombyx mori silk, silk fibroin, adipic acid, calcitonin gene-related peptide, electrostatic interaction

中图分类号:

TS101.4

宋广州, 涂芳芳, 丁梦瑶, 戴梦男, 殷音, 董凤林, 王建南. 丝素蛋白负电性增强改性及其对降钙素基因相关肽的加载能力[J]. 纺织学报, 2020, 41(12): 7-12.

SONG Guangzhou, TU Fangfang, DING Mengyao, DAI Mengnan, YIN Yin, DONG Fenglin, WANG Jiannan. Negatively enhanced modification of silk fibroin and its load ability to calcitonin gene-related peptide[J]. Journal of Textile Research, 2020, 41(12): 7-12.

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参考文献 20

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丝素蛋白负电性增强改性及其对降钙素基因相关肽的加载能力

Negatively enhanced modification of silk fibroin and its load ability to calcitonin gene-related peptide

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