纺织学报 ›› 2022, Vol. 43 ›› Issue (04): 15-19.doi: 10.13475/j.fzxb.20210302305

• 纤维材料 • 上一篇    下一篇

不同水解方式下蚕丝丝素蛋白材料的止血性能

雷彩虹1,2, 俞林双1, 朱海霖1,2, 郑涛1, 陈建勇1,2()   

  1. 1.浙江理工大学 纺织科学与工程学院(国际丝绸学院), 浙江 杭州 310018
    2.浙江省纤维材料和加工技术研究重点实验室, 浙江 杭州 310018
  • 收稿日期:2021-03-05 修回日期:2022-01-19 出版日期:2022-04-15 发布日期:2022-04-20
  • 通讯作者: 陈建勇
  • 作者简介:雷彩虹(1975—),女,讲师,博士。主要研究方向为丝素生物材料的结构和性能。
  • 基金资助:
    浙江省基础公益研究计划项目(LGC21E030003);浙江省教育厅一般科研项目(20200066-F);国家自然科学基金项目(51273181);浙江理工大学科研启动项目(19202448-Y)

Hemostasis properties of silk fibroin materials under different types of hydrolysis

LEI Caihong1,2, YU Linshuang1, ZHU Hailin1,2, ZHENG Tao1, CHEN Jianyong1,2()   

  1. 1. College of Textile Science and Engineering(International Institute of Silk), Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
    2. Zhejiang Provincial Key Laboratory of Fiber Materials and Manufacturing Technology, Hangzhou, Zhejiang 310018, China
  • Received:2021-03-05 Revised:2022-01-19 Published:2022-04-15 Online:2022-04-20
  • Contact: CHEN Jianyong

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摘要：

为提升蚕丝丝素蛋白材料的止血性能,采用盐解和酶解2种方式水解蚕丝丝素蛋白,通过冷冻干燥法制备得到2种蚕丝丝素蛋白材料。通过凝胶过滤色谱、水溶性实验、凝血因子实验、血小板黏附实验、酶联免疫吸附实验等分析了2种蚕丝丝素蛋白材料的分子质量、水溶性和止血效果。结果表明:与盐解法相比,酶解所得材料具有较低的分子质量和较好的水溶性,有利于激活凝血因子Ⅻ从而对内源性凝血起到促进作用,止血时血小板黏附量和血小板第4因子含量会明显增加,大鼠肝部出血量减少且出血时间缩短,其止血效果优于市售云南白药。

关键词: 蚕丝, 丝素蛋白, 水解方式, 盐解, 酶解, 止血性能

Abstract:

Aiming at the improvement of hemostasis properties of silk fibroin materials fabricated by salt hydrolysis and enzyme hydrolysis method, the silk fibroin materials were prepared via a freeze-drying method. The molecular weight, water solubility and hemostatic effect of the resultant material were analyzed by gel filtration chromatography, water solubility test, coagulation factor test, platelet adhesion test, enzyme-linked immunosorbent assay and rat liver injury model. The results showed that compared with the salt hydrolysis method, the enzyme hydrolysis method could endow a lower molecular weight and better water soluble, which is conducive to activating the coagulation factor XII to promote endogenous coagulation. Moreover, the platelet adhesion and platelet factor 4 content increase significantly, the amount of bleeding from the rat liver is reduced with shortened bleeding time, indicating better hemostatic effect than that of Yunnan white medicine.

Key words: silk, silk fibroin, types of hydrolysis, salt hydrolysis, enzyme hydrolysis, hemostasis property

中图分类号: 

  • TQ341.5

图1

采用2种水解方式制备的蚕丝丝素蛋白材料的APTT与PT值"

图2

2种水解方式下制备的蚕丝丝素蛋白材料的吸光度"

图3

2种水解方式下制备的蚕丝丝素蛋白材料肝部出血时间和出血量"

[1] YANG X, LIU W, LI N, et al. Design and development of polysaccharide hemostatic materials and their hemostatic mechanism[J]. Biomaterials Science, 2017, 5(12): 2357-2368.
doi: 10.1039/C7BM00554G
[2] 钱璐敏, 张斌. 可溶性止血医用棉纱布的制备及其性能[J]. 纺织学报, 2019, 40(5):102-106.
QIAN Lumin, ZHANG Bin. Preparation and characterization of soluble hemostatic medical cotton gauze[J]. Journal of Textile Research, 2019, 40(5):102-106.
[3] BANDYOPADHYAY A, CHOWDHURY S K, DEY S, et al. Silk: a promising biomaterial opening new vistas towards affordable healthcare solutions[J]. Journal of the Indian Institute of Science, 2019, 99(3): 445-487.
doi: 10.1007/s41745-019-00114-y
[4] 严佳, 李刚. 医用纺织品的研究进展[J]. 纺织学报, 2020, 41(9):191-200.
YAN Jia, LI Gang. Research progress on medical textiles[J]. Journal of Textile Research, 2020, 41(9):191-200.
[5] 钟红荣, 方艳, 包红, 等. 丝素基双层敷料的制备及其性能[J]. 纺织学报, 2020, 41(2):13-19.
ZHONG Hongrong, FANG Yan, BAO Hong, et al. Preparation and properties of silk fibroin based bilayer dressing materials[J]. Journal of Textile Research, 2020, 41(2):13-19.
[6] CHEN G, WE R, HUANG X, et al. Synthesis and assessment of sodium alginate-modified silk fibroin microspheres as potential hepatic arterial embolization agent[J]. International Journal of Biological Macromolecules, 2020, 155: 1450-1459.
doi: 10.1016/j.ijbiomac.2019.11.122
[7] HUANG X, FU Q, DENG Y, et al. Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo[J]. Carbohydrate Polymers, 2021, 253: 117256.
doi: 10.1016/j.carbpol.2020.117256
[8] ZHOU F, XUE Z, WANG J. Antihypertensive effects of silk fibroin hydrolysate by alcalase and purification of an ACE inhibitory dipeptide[J]. Journal of Agricultural and Food Chemistry, 2010, 58(11):6735-6740.
doi: 10.1021/jf101101r
[9] 郭少哲. 基于结构和分子量调控的丝素蛋白纯化方法的研究和应用[D]. 苏州: 苏州大学, 2016:11-12.
GUO Shaozhe. Insight into structure and molecular weight of silk fibroin for research and application[D]. Suzhou: Soochow University, 2016:11-12.
[10] LAN G, LU B, WANG T, et al. Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent[J]. Colloids & Surfaces B: Biointerfaces, 2015, 136:1026-1034.
[11] 程玮璐. 天然高分子基多功能止血复合敷料的制备及其性能研究[D]. 哈尔滨: 哈尔滨工业大学, 2016:35,60.
CHENG Weilu. Study on preparation and performance of natural polymer-based functional composite for hemostatic wound dressing[D]. Harbin: Harbin Institute of Technology, 2016:35,60.
[12] WU J, LEMARIÉ C A, BARRALET J, et al. Amphiphilic peptide-loaded nanofibrous calcium phosphate microspheres promote hemostasis in vivo[J]. Acta Biomaterialia, 2013, 9(11): 9194-9200.
doi: 10.1016/j.actbio.2013.06.023
[13] AVANTIKA G, HARIT P, MATTHEW R S, et al. Enzymes produced by autoactivation of blood factor XII in buffer: a contribution from the Hematology at Biomaterial Interfaces Research Group[J]. Biomaterials, 2015, 37: 1-12.
doi: 10.1016/j.biomaterials.2014.09.015
[14] TOULON P, ELOIT Y, SMAHI M, et al. In vitro sensitivity of different activated partial thromboplastin time reagents to mild clotting factor deficiencies[J]. International Journal of Laboratory Hematology, 2016, 38(4):389-396.
doi: 10.1111/ijlh.12499
[15] 王会敏, 潘吉勇, 杨冰, 等. 全溶性止血纤维的动物试验及临床应用研究[J]. 中国临床药理学与治疗学, 2006, 11(9): 1017-1020.
WANG Huimin, PAN Jiyong, YANG Bing, et al. Study of animal and clinical use on fully soluble hemo-static[J]. Chinese Clinical Pharmacology and Therapeutics, 2006, 11(9): 1017-1020.
[16] SMITH C W, ZAHER R, LOLA P, et al. TREM-like transcript 1: a more sensitive marker of platelet activation than P-selectin in humans and mice[J]. Blood Advances, 2018, 2(16): 2072-2078.
doi: 10.1182/bloodadvances.2018017756
[17] KUNDU B, SCHLIMP C J, NÜRNBERGER S, et al. Thromboelastometric and platelet responses to silk biomaterials[J]. Scientific Reports, 2014, 4(1): 2941-2953.
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