纺织学报 ›› 2021, Vol. 42 ›› Issue (01): 35-39.doi: 10.13475/j.fzxb.20200405405

• 纤维材料 • 上一篇    下一篇

羊毛角蛋白在巯基乙酸胆碱中的溶解再生

袁久刚(), 季吉, 薛琪, 姜哲, 范雪荣, 高卫东   

  1. 生态纺织教育部重点实验室(江南大学), 江苏 无锡 214122
  • 收稿日期:2020-04-10 修回日期:2020-09-28 出版日期:2021-01-15 发布日期:2021-01-21
  • 作者简介:袁久刚(1982—),男,副教授,博士。主要研究方向为天然蛋白质材料提取及改性。E-mail: jiugangyuan@jiangnan.edu.cn。
  • 基金资助:
    国家自然科学基金项目(31771039);国家重点研发计划专项资助项目(2017YFB0309200);中央高校基本科研业务费专项资金项目(JUSRP51717A);江苏省高等学校大学生创新创业训练计划项目(201910295064Z)

Dissolution and regeneration of wool keratin in choline thioglycolate

YUAN Jiugang(), JI Ji, XUE Qi, JIANG Zhe, FAN Xuerong, GAO Weidong   

  1. Key Laboratory of Eco-Textiles (Jiangnan University), Ministry of Education, Wuxi, Jiangsu 214122, China
  • Received:2020-04-10 Revised:2020-09-28 Online:2021-01-15 Published:2021-01-21

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摘要：

为提高废弃羊毛纤维的综合利用率,采用一种新型的还原性类离子液体巯基乙酸胆碱对羊毛进行溶解和再生,观察了羊毛纤维在其中的溶解过程。采用红外光谱仪、X射线衍射分析仪、差示扫描量热仪以及凝胶电泳仪对不同温度下提取的再生角蛋白性能进行了研究。结果表明:巯基乙酸胆碱能够有效打开羊毛角蛋白大分子之间的二硫键,从而实现对羊毛皮质层及鳞片层的高效溶解,120 ℃条件下,最高溶解质量分数可以达到16%;经巯基乙酸胆碱溶解再生制得的角蛋白,其蛋白主体结构得到保留,但是与原羊毛相比,再生角蛋白中的α-螺旋含量降低,分子质量降低,并且随着溶解温度提高,角蛋白大分子也发生了一定程度降解。

关键词: 羊毛, 角蛋白, 巯基乙酸胆碱, 离子液体, 二硫键

Abstract:

In order to improve the solubility and utilization of waste wool fiber, a new type of reducing ionic liquid-choline thioglycolate, was employed for wool dissolution in the reported research. The dissolution process of a single wool fibre in choline thioglycolate was carefully observed using polarizing optical microscope. The wool keratin extracted at different temperatures was characterized through fourier transform infrared spectrometer,X-ray diffraction,differential scanning calorimetry and SDS-PAGE in detail. The results show that choline thioglycolate is able to effectively destroy the disulfide bonds in wool keratin, thus achieving efficient dissolution of the wool cortex and scales. At 120 ℃, the dissolution of wool in choline thioglycolate reached up to 16%, and the regenerated wool keratin retained the main body structure. However, compared with raw wool fibers, the α-helix content in the regenerated keratin and the molecular weight decreased, and the keratin macromolecules degraded seriously under higher temperature.

Key words: wool, keratin, choline thioglycolate, ionic liquid, disulfide bond

中图分类号: 

  • TS195.5

图1

羊毛在巯基乙酸胆碱中的溶解速率曲线"

图2

羊毛纤维在巯基乙酸胆碱中溶解过程"

图3

皮质层在巯基乙酸胆碱中溶解过程"

图4

鳞片层在巯基乙酸胆碱中溶解过程"

图5

原羊毛及再生角蛋白红外光谱图"

图6

羊毛及再生角蛋白的X射线衍射图谱"

表1

羊毛及再生角蛋白结晶指数"

样品 溶解温度/℃ I9/a.u. I14/a.u. CI
原毛 592 278 0.53
再生角蛋白 110 340 224 0.34
再生角蛋白 120 380 266 0.30
再生角蛋白 130 388 291 0.25

图7

再生角蛋白差示扫描量热图"

图8

羊毛和再生角蛋白的SDS-PAGE图 a—标准蛋白;b—再生角蛋白(110 ℃); c—再生角蛋白(120 ℃);d—再生角蛋白(130 ℃);e—原羊毛。"

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