壳聚糖基抑菌凝胶剂的制备及其性能

doi:10.13475/j.fzxb.20210305707

纺织学报 ›› 2022, Vol. 43 ›› Issue (03): 71-77.doi: 10.13475/j.fzxb.20210305707

壳聚糖基抑菌凝胶剂的制备及其性能

张涛¹, 王富平¹, 陈国宝¹, 吴基玉², 庞亚妮², 陈忠敏¹()

1.重庆理工大学药学与生物工程学院, 重庆 400054
2.重庆华伦医疗器械有限公司, 重庆 400039

收稿日期:2021-03-15 修回日期:2021-10-17 出版日期:2022-03-15 发布日期:2022-03-29
通讯作者: 陈忠敏
作者简介:张涛(1996—),男,硕士生。主要研究方向为生物材料和功能性高分子材料。
基金资助:
重庆市科技局社会民生与保障专项项目(cstc2016shmszx130025)

Preparation and performance of chitosan-based antibacterial gel

ZHANG Tao¹, WANG Fuping¹, CHEN Guobao¹, WU Jiyu², PANG Yani², CHEN Zhongmin¹()

1. College of Pharmacy and Biological Engineering, Chongqing University of Technology, Chongqing 400054, China
2. Chongqing Hualun Medical Equipment Co., Ltd., Chongqing 400039, China

Received:2021-03-15 Revised:2021-10-17 Published:2022-03-15 Online:2022-03-29
Contact: CHEN Zhongmin

摘要/Abstract

摘要：

针对壳聚糖-甘油磷酸钠体系黏度低、抑菌效果差等问题,以壳聚糖-甘油磷酸钠体系为基材,加入羧甲基纤维素钠调节黏度后,分别加入丝胶蛋白和丝素蛋白以共混方式确定最优组合制备复合凝胶剂,对其抑菌效果、各组分结合情况、抑菌机制、细胞毒性和皮肤刺激性进行分析。结果表明:在壳聚糖(质量分数为3%)和甘油磷酸钠(质量分数为50%)体积比为9∶20的体系中加入质量分数为1.5%的羧甲基纤维素钠后,其外观良好,均匀无颗粒感,适宜外用;当添加丝胶蛋白质量分数为15%时,复合凝胶剂的抑菌效果最优,对大肠杆菌和金黄色葡萄球菌的抑菌率分别为(96.20±3.40)%和(86.30±2.51)%;复合凝胶剂结构稳定,可使细菌细胞膜通透性改变,导致细菌无法增殖而发挥抑菌作用,且无细胞毒性和皮肤刺激性。

关键词: 壳聚糖, 丝胶蛋白, 丝素蛋白, 复合凝胶剂, 抑菌性

Abstract:

This research addresses the problems of low viscosity and poor antibacterial effect of the chitosan-sodium glycerophosphate system. The chitosan-sodium glycerophosphate system was used as the base material. After sodium carboxymethylcellulose was added to adjust the viscosity, sericin and silk fibroin were added separately to determine the optimal combination to prepare a composite gel. The antibacterial effect, the combination of each component, the antibacterial mechanism, cytotoxicity, and skin irritation of the composite gel were analyzed. The results show that the mixture of the 3% chitosan and 50% sodium glycerophosphate in the volume ratio of 9∶20 together with 1.5% sodium carboxymethyl cellulose demonstrates the moderate viscosity of (68 300±540) mPa·s, which was suitable for external use. When the mass fraction of sericin is 15%, the antibacterial effect of the composite gel is the best, and the antibacterial rate against Escherichia coli and Staphylococcus aureus is (96.20±3.40)% and (86.30±2.51)%, respectively. The gel has a stable structure, and it was found that the composite gel changes the permeability of the bacterial cell membrane, resulting in the inability of the bacteria to proliferate and antibacterial effects, without cytotoxicity and skin irritation.

Key words: chitosan, sericin, silk fibroin, composite gel, antimicrobial

中图分类号:

TQ341.5

张涛, 王富平, 陈国宝, 吴基玉, 庞亚妮, 陈忠敏. 壳聚糖基抑菌凝胶剂的制备及其性能[J]. 纺织学报, 2022, 43(03): 71-77.

ZHANG Tao, WANG Fuping, CHEN Guobao, WU Jiyu, PANG Yani, CHEN Zhongmin. Preparation and performance of chitosan-based antibacterial gel[J]. Journal of Textile Research, 2022, 43(03): 71-77.

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试样名称	浸提液质量分数/%	OD值	细胞膜透过率/%
浸提液+细菌悬液+PI	25	0.222±0.025	23.67
	50	0.305±0.018	31.47
	75	0.431±0.031	45.94
	100	0.546±0.036	58.17
氨苄青霉素+细菌悬液 +PI	—	0.601±0.039	64.07
Triton X-100+细菌悬液+PI	—	0.938±0.052	100
缓冲液+ 细菌悬液+PI	—	0	0
浸提液+PI	100	0	0

壳聚糖基抑菌凝胶剂的制备及其性能

Preparation and performance of chitosan-based antibacterial gel

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