Journal of Textile Research ›› 2025, Vol. 46 ›› Issue (10): 30-38.doi: 10.13475/j.fzxb.20250200301

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Preparation and performance of antibacterial nanofiber membrane loaded with magnolol

WU Leran1, WU Nihuan2, LI Lingeng1, ZHONG Yi1, CHEN Hongpeng2, TANG Nan1()   

  1. 1. School of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, China
    2. School of Biomedical Engineering, Guangdong Medical University, Dongguan, Guangdong 523808, China
  • Received:2025-02-05 Revised:2025-06-19 Online:2025-10-15 Published:2025-10-15
  • Contact: TANG Nan E-mail:tn6559@foxmail.com

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Abstract:

Objective To overcome the limitations of traditional wound dressings such as poor antibacterial function and the need for frequent replacement, it was necessary to develop innovative multifunctional wound dressings. In this study, a nanofiber membrane loaded with magnolol (MAG) was fabricated using electrospinning technology. The membrane's morphology, wettability, water vapor transmission rate, mechanical properties, drug release, biocompatibility, hemocompatibility, antioxidant activity and antibacterial efficacy were systematically evaluated to explore its potential application as a wound dressing.

Method MAG was dissolved in varying mass ratios of zein/gelatin (Zein/Gel) solutions to prepare precursor solutions for electrospinning. Zein/Gel/MAG composite nanofiber membranes were then fabricated. The nanofibers' microstructure, chemical composition, and wettability were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy (FT-IR) and contact angle measurements. The effects of different Zein/Gel mass ratios on water vapor transmission rate, mechanical properties, MAG release, cytotoxicity, hemocompatibility, antioxidant activity and antibacterial efficacy were also investigated.

Results The fabricated Zein/Gel/MAG nanofiber membranes displayed a smooth surface without obvious bead formation or adhesion, and the nanofiber diameter decreased as the mass proportion of Gel in the spinning solution declined. FT-IR analysis confirmed the presence of Zein, Gel and MAG in the nanofibers. Water contact angle measurements indicated that the nanofiber membranes were hydrophobic, with increasing hydrophobicity accompanying the reduction in the mass proportion of Gel, reaching a maximum value of (116.50± 9.24)°. All nanofiber membranes demonstrated water vapor transmission rates above (2 527.42±262.94) g/(m2·d), reaching a maximum value of (2 805.50±65.17) g/(m2·d).The cumulative release of MAG from nanofiber membranes was strongly correlated with the mass proportion of Gel. Furthermore, the Zein/Gel/MAG nanofiber membranes exhibited no significant cytotoxicity to L929 cells and hemolysis rates of less than 2%. The scavenging rates for 1,1-diphenyl-2-picrylhydrazine free radical were in the range of(32.93±2.22)% to (53.03±8.32)%. The maximum widths of bacteriostatic circle for S.aureus and E.coli were (1.89±0.62) mm and (1.80±0.06) mm, respectively.

Conclusion The Zein/Gel/MAG nanofiber membranes were successfully prepared via electrospinning. These membranes exhibited excellent water vapor transmission rate, biocompatibility and hemocompatibility, as well as remarkable antioxidant activity. Specially, the obvious antibacterial efficacies against S.aureus and E.coli were observed. Therefore, the Zein/Gel/MAG nanofiber membrane had significant potential prospect for wound dressings.

Key words: magnolol, zein, gelatin, nanofiber membrane, antibacterial, wound dressing, electrospinning

CLC Number: 

  • TQ340.64

Fig.1

Morphology photo of Zein/Gel/MAG nanofiber membranes"

Fig.2

Histogram of nanofiber diameter distribution"

Tab.1

Viscosity value of spinning solution"

纺丝溶液 黏度/(mPa·s)
Zein/Gel/MAG-1 1 545.5
Zein/Gel/MAG-2 1 321.2
Zein/Gel/MAG-3
Zein/Gel/MAG-4		 869.7
561.8

Fig.3

Infrared spectrogram of Gel, MAG, Zein and Zein/Gel/MAG-1"

Fig.4

Water contact angle of Zein/Gel/MAG nanofiber membranes"

Fig.5

Stress-strain curves of Zein/Gel/MAG nanofiber membranes"

Fig.6

MAG cumulative release curves from Zein/Gel/MAG nanofiber membranes"

Fig.7

Effect of Zein/Gel/MAG nanofiber membranes extract on viability of L929 cells"

Fig.8

Photographic image (a) and hemolysis rate (b) of Zein/Gel/MAG nanofiber membranes hemolysis"

Fig.9

Antioxidation of Zein/Gel/MAG nanofiber membranes"

Fig.10

Antibacterial effects of nanofiber membranes. (a) To S. aureus; (b) To E. coli"

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