Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (02): 125-131.doi: 10.13475/j.fzxb.20210902007

• Textile Engineering • Previous Articles     Next Articles

Fabrication of novel biodegradable braided nerve grafts for nerve regeneration

YAO Ruotong1,2, ZHAO Jingyuan1, YAN Yixin1, DUAN Lirong1,2, WANG Tian3, YAN Jia1,2, ZHANG Shujun1, LI Gang1,2()   

  1. 1. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
    2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China
    3. Wilson College of Textiles, North Carolina State University, Raleigh 27695, USA
  • Received:2021-09-07 Revised:2021-11-21 Online:2022-02-15 Published:2022-03-15
  • Contact: LI Gang E-mail:tcligang@suda.edu.cn

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Abstract:

In order to develop artificial nerve guidance conduits (NGCs) with good mechanical properties and biocompatibility, this paper describes a three-layer composite artificial nerve graft made up with a chitosan coating layer, a braided layer and a fibrous sponge layer using traditional braiding, electrospinning and freeze-drying techniques. The morphology, mechanical properties, biocompatibility and sustained-release properties were investigated, studying the effects of axial yarn, the outer and inner layers and magnesium ion concentration on its performance. The results showed that the prepared NGCs with braided and axial yarns possess good mechanical properties. The radial compressive property of the NGC with 50% deformation is 1.3 N and the axial tensile stress to yarn fracture is 30 N. The sponge layer features an inter-connected porous structure with uniform pore size distribution (0.04-0.08 mm). The magnesium ion in the NGC can be sustainably released for 28 d. When the concentration of magnesium ion solution is 0.02 g/mL, the NGC has the greatest effect on promoting cell proliferation. This paper provides new ideas for selecting materials for NGCs and optimizing their structural properties.

Key words: artificial nerve graft, peripheral nerve repair, biodegradability, silk fibroin, magnesium ion

CLC Number: 

  • TS102.3

Tab.1

Preparation parameters of nerve guidance conduits"

编号 编织纱线密度/tex 轴纱线密度/tex 涂层 内层
1 9 5 壳聚糖 海绵层
2 9 — 壳聚糖 海绵层
3 9 5 — —
4 9 — — —

Fig.1

Morphology and diameter distribution of electrospun nanofibers"

Tab.2

Element content of electrospun nanofiber membranes with different magnesium ion concentrations"

样品中M g 2 +质量
浓度/(g·mL-1)		 元素 质量分数/% 均方差 原子含量/%
0 C 67.152 0.680 73.143
O 32.838 0.679 26.852
Mg 0.010 0.062 0.006
0.01 C 69.003 0.607 74.850
O 30.666 0.608 24.972
Mg 0.310 0.067 0.177
0.015 C 69.197 0.607 75.013
O 30.509 0.608 24.829
Mg 0.294 0.068 0.158
0.02 C 67.662 0.611 73.790
O 31.390 0.613 25.699
Mg 0.948 0.079 0.511

Tab.3

FT-IR characteristic peaks of silk fibroin"

结构名称 浓度/cm-1 结构名称 浓度/cm-1
酰胺 Ⅰ 1 650~1 660 酰胺 Ⅰ 1 625~1 640
Silk Ⅰ 酰胺 Ⅱ 1 535~1 545 Silk Ⅱ 酰胺 Ⅱ 1 515~1 525
酰胺 Ⅲ 1 253 酰胺 Ⅲ 1 235

Fig.2

FT-IR curves of electrospun nanofiber membranes"

Fig.3

SEM micrograghs for braided nerve guidance conduits. (a) Cross section of conduit without sponge core; (b) Cross section of conduit with sponge core; (c) Wall of conduit"

Fig.4

Pore size distribution of nanofiber spongy core in nerve guidance conduit"

Fig.5

Mechanical properties of nerve guidance conduits. (a) Radial compression property; (b) Axial tensile property"

Fig.6

Release curves of magnesium ion in nerve guidance conduits"

Fig.7

CCK-8 results of cells cultured in conduits"

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