Pineapple leaf fibers are thick, long, straight, and with branches on the surface, leading to poor quality of pure spun yarn. In order to improve its spinnability, the silk spinning system was employed to blend pineapple leaf fiber with some biomass fibers to prepare 16.7 tex×2 two-component yarns and 14.3 tex×2 three-component yarns, the selected biomass fibers including spun silk, wool, chitosan fiber, Lyocell fiber and polylactic acid (PLA) fiber. The mechanical properties, evenness and hairiness of the yarns were evaluated, and the results show that the spun yarns have high breaking strength, good evenness and low hairiness, which can be used to develop medium to high grade woven fabrics and can be applied to clothing making. Among the two-component blended yarns, the performance of pineapple leaf fiber/spun silk (mass ratio:85∶15) is the best, with breaking strength being(21.9±2.3)cN/tex, the hairiness value 3.61 mm/cm, and the evenness CV value 18.05%. Among the three-component blended yarns, the performance of pineapple leaf fiber/spun silk/Lyocell fiber (mass ratio:50∶30∶20) is the best, whose breaking strength is(19.9±1.3)cN/tex, the hairiness value 3.26 mm/cm, and the evenness CV value 14.03%.